Ishak, M. I., Eales, M., Damiati, L., Liu, X., Jenkins, J., Dalby, M. J. , Nobbs, A. H., Ryadnov, M. G. and Su, B. (2023) Enhanced and stem-cell-compatible effects of nature-inspired antimicrobial nanotopography and antimicrobial peptides to combat implant-associated infection. ACS Applied Nano Materials, 6(4), pp. 2549-2559. (doi: 10.1021/acsanm.2c04913) (PMCID:PMC9972347)
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Abstract
Nature-inspired antimicrobial surfaces and antimicrobial peptides (AMPs) have emerged as promising strategies to combat implant-associated infections. In this study, a bioinspired antimicrobial peptide was functionalized onto a nanospike (NS) surface by physical adsorption with the aim that its gradual release into the local environment would enhance inhibition of bacterial growth. Peptide adsorbed on a control flat surface exhibited different release kinetics compared to the nanotopography, but both surfaces showed excellent antibacterial properties. Functionalization with peptide at micromolar concentrations inhibited Escherichia coli growth on the flat surface, Staphylococcus aureus growth on the NS surface, and Staphylococcus epidermidis growth on both the flat and NS surfaces. Based on these data, we propose an enhanced antibacterial mechanism whereby AMPs can render bacterial cell membranes more susceptible to nanospikes, and the membrane deformation induced by nanospikes can increase the surface area for AMPs membrane insertion. Combined, these effects enhance bactericidal activity. Since functionalized nanostructures are highly biocompatible with stem cells, they make promising candidates for next generation antibacterial implant surfaces.
Item Type: | Articles |
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Additional Information: | This work received funding from the BBSRC iCASE Studentship (EGM) (1723473), MRC (MR/S010343/1), and EU (BioTUNE). Bio-TUNE project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 872869. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Dalby, Professor Matthew |
Authors: | Ishak, M. I., Eales, M., Damiati, L., Liu, X., Jenkins, J., Dalby, M. J., Nobbs, A. H., Ryadnov, M. G., and Su, B. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | ACS Applied Nano Materials |
Publisher: | American Chemical Society |
ISSN: | 2574-0970 |
ISSN (Online): | 2574-0970 |
Published Online: | 15 February 2023 |
Copyright Holders: | Copyright © 2023 The Authors |
First Published: | First published in ACS Applied Nano Materials 6(4): 2549-2559 |
Publisher Policy: | Reproduced under a Creative Commons License |
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