Altinli, M., Leggewie, M., Schulze, J., Gyanwali, R., Badusche, M., Sreenu, V. B. , Fuss, J. and Schnettler, E. (2023) Antiviral RNAi response in Culex quinquefasciatus-derived HSU cells. Viruses, 15(2), 436. (doi: 10.3390/v15020436) (PMID:36851650) (PMCID:PMC9968050)
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Abstract
Culex spp. mosquitoes are important vectors of viruses, such as West Nile virus, Eastern equine encephalitis virus and Rift valley fever virus. However, their interactions with innate antiviral immunity, especially RNA interference (RNAi), are not well known. Most research on RNAi pathways in mosquitoes is focused on the tropical vector mosquito Aedes aegypti. Here, we investigated the production of arbovirus-specific small RNAs in Cx. quinquefasciatus-derived HSU cells. Furthermore, by silencing RNAi-related proteins, we investigated the antiviral role of these proteins for two different arboviruses: Semliki Forest virus (SFV) and Bunyamwera orthobunyavirus (BUNV). Our results showed an expansion of Ago2 and Piwi6 in Cx. quinquefasciatus compared to Ae. aegypti. While silencing Ago2a and Ago2b increased BUNV replication, only Ago2b showed antiviral activity against SFV. Our results suggest differences in the function of Cx. quinquefasciatus and Ae. aegypti RNAi proteins and highlight the virus-specific function of these proteins in Cx. quinquefasciatus.
Item Type: | Articles |
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Keywords: | RNA interference, Culex quinquefasciatus, Aedes aegypti, RNAi, antiviral immunity. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Vattipally, Dr Sreenu |
Creator Roles: | |
Authors: | Altinli, M., Leggewie, M., Schulze, J., Gyanwali, R., Badusche, M., Sreenu, V. B., Fuss, J., and Schnettler, E. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Viruses |
Publisher: | MDPI |
ISSN: | 1999-4915 |
ISSN (Online): | 1999-4915 |
Published Online: | 04 February 2023 |
Copyright Holders: | Copyright © 2023 The Authors |
First Published: | First published in Viruses 15(2): 436 |
Publisher Policy: | Reproduced under a Creative Commons License |
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