Evaluation of an engineered Zika virus-like particle vaccine candidate in a mosquito-mouse transmission model

Mancini, M. V. et al. (2023) Evaluation of an engineered Zika virus-like particle vaccine candidate in a mosquito-mouse transmission model. mSphere, 8(2), e00564-22. (doi: 10.1128/msphere.00564-22) (PMID:36840596) (PMCID:PMC10117074)

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Abstract

The primary route of Zika virus (ZIKV) transmission is through the bite of an infected Aedes mosquito, when it probes the skin of a vertebrate host during a blood meal. Viral particles are injected into the bite site together with mosquito saliva and a complex mixture of other components. Some of them are known to play a key role in the augmentation of the arbovirus infection in the host, with increased viremia and/or morbidity. This vector-derived contribution to the infection is not usually considered when vaccine candidates are tested in preclinical animal models. In this study, we performed a preclinical validation of a promising ZIKV vaccine candidate in a mosquito-mouse transmission model using both Asian and African ZIKV lineages. Mice were immunized with engineered ZIKV virus-like particles and subsequently infected through the bite of ZIKV-infected Aedes aegypti mosquitoes. Despite a mild increase in viremia in mosquito-infected mice compared to those infected through traditional needle injection, the vaccine protected the animals from developing the disease and strongly reduced viremia. In addition, during peak viremia, naive mosquitoes were allowed to feed on infected vaccinated and nonvaccinated mice. Our analysis of viral titers in mosquitos showed that the vaccine was able to inhibit virus transmission from the host to the vector.

Item Type:Articles
Additional Information:This research was funded by the Department of Health and Social Care using UK Aid funding and is managed by the NIHR, and by the UK Medical Research Council, MC_UU12014/2 for AHP and MC_UU12014/8 for AK. This project was also partially funded through the Wellcome Trust award 202888/Z/16/Z (SPS).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:De Lorenzo, Dr Giuditta and Murdochy, Mr Shivan and Ant, Dr Thomas and Gingell, Mr Daniel and Setthapramote, Dr Chayanee and Patel, Professor Arvind and Sinkins, Professor Steven and Mancini, Dr Maria Vittoria and Tandavanitj, Mr Rapeepat and NATSRITA, PIYATIDA and Kohl, Professor Alain
Creator Roles:
Mancini, M. V.Conceptualization, Methodology, Validation, Formal analysis, Investigation, Data curation, Writing – original draft, Writing – review and editing, Visualization, Supervision, Project administration
Tandavanitj, R.Methodology, Validation, Formal analysis, Investigation, Data curation, Writing – review and editing
Ant, T.Methodology, Validation, Formal analysis, Investigation, Data curation, Writing – review and editing
Murdochy, S.Methodology, Investigation, Writing – review and editing
Gingell, D.Methodology, Validation, Formal analysis, Investigation, Data curation, Writing – review and editing
Setthapramote, C.Methodology, Investigation, Writing – review and editing
NATSRITA, P.Methodology, Investigation, Writing – review and editing
Kohl, A.Resources, Writing – review and editing
Sinkins, S.Resources, Writing – review and editing, Funding acquisition
Patel, A.Resources, Writing – review and editing, Project administration, Funding acquisition
De Lorenzo, G.Conceptualization, Methodology, Validation, Formal analysis, Investigation, Data curation, Writing – original draft, Writing – review and editing, Visualization, Supervision, Project administration, Funding acquisition
Authors: Mancini, M. V., Tandavanitj, R., Ant, T. H., Murdochy, S. M., Gingell, D. D., Setthapramote, C., Natsrita, P., Kohl, A., Sinkins, S. P., Patel, A. H., and De Lorenzo, G.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:mSphere
Publisher:American Society for Microbiology
ISSN:2379-5042
ISSN (Online):2379-5042
Published Online:22 February 2023
Copyright Holders:Copyright © 2023 Mancini et al.
First Published:First published in mSphere 8(2):e00564-22
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172630Basis of the host range and tissue tropism for hepatitis C virusArvind PatelMedical Research Council (MRC)MC_UU_12014/2III - Centre for Virus Research
172630007Arthropod-borne infections and emerging virus infections in high risk areas (Programme 4)Alain KohlMedical Research Council (MRC)MC_UU_12014/8III - Centre for Virus Research
173477Wolbachia-mediated arbovirus inhibition in mosquitoesSteven SinkinsWellcome Trust (WELLCOTR)202888/Z/16/ZIII-MRC-GU Centre for Virus Research