Photobacterium profundum under pressure: A MS-based label-free quantitative proteomics study

Le Bihan, T., Rayner, J., Roy, M. M. and Spagnolo, L. (2013) Photobacterium profundum under pressure: A MS-based label-free quantitative proteomics study. PLoS ONE, 8(5), e60897. (doi: 10.1371/journal.pone.0060897) (PMID:23741291) (PMCID:PMC3669370)

Abstract

Photobacterium profundum SS9 is a Gram-negative bacterium, originally collected from the Sulu Sea. Its genome consists of two chromosomes and a 80 kb plasmid. Although it can grow under a wide range of pressures, P. profundum grows optimally at 28 MPa and 15°C. Its ability to grow at atmospheric pressure allows for both easy genetic manipulation and culture, making it a model organism to study piezophily. Here, we report a shotgun proteomic analysis of P. profundum grown at atmospheric compared to high pressure using label-free quantitation and mass spectrometry analysis. We have identified differentially expressed proteins involved in high pressure adaptation, which have been previously reported using other methods. Proteins involved in key metabolic pathways were also identified as being differentially expressed. Proteins involved in the glycolysis/gluconeogenesis pathway were up-regulated at high pressure. Conversely, several proteins involved in the oxidative phosphorylation pathway were up-regulated at atmospheric pressure. Some of the proteins that were differentially identified are regulated directly in response to the physical impact of pressure. The expression of some proteins involved in nutrient transport or assimilation, are likely to be directly regulated by pressure. In a natural environment, different hydrostatic pressures represent distinct ecosystems with their own particular nutrient limitations and abundances. However, the only variable considered in this study was atmospheric pressure.

• Item Type: Articles
• Additional Information: LS acknowledges funding by a Leverhulme Trust grant awarded to the Centre for Science at Extreme Conditions (reference F/00 158/V). TLB is funded by the SynthSys Edinburgh is a Centre for Integrative Systems Biology (CISB) funded by BBSRC and EPSRC, reference BB/D019621/1. MMR was supported by a grant from the European Research Council (233457-SCG) to M. Tyers.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Spagnolo, Professor Laura
• Authors: Le Bihan, T., Rayner, J., Roy, M. M., and Spagnolo, L.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: PLoS ONE
• Publisher: Public Library of Science
• ISSN: 1932-6203
• ISSN (Online): 1932-6203
• Copyright Holders: Copyright: © 2013 Le Bihan et al.
• First Published: First published in PLoS ONE 8(5)
• Publisher Policy: Reproduced under a Creative Commons licence