Ayers, V. B., Huang, Y.-J. S., Kohl, A. , Dunlop, J. I., Hettenbach, S. M., Park, S. L., Higgs, S. and Vanlandingham, D. L. (2023) Comparison of immunogenicity between a candidate live attenuated vaccine and an inactivated vaccine for Cache Valley virus. Viral Immunology, 36(1), pp. 41-47. (doi: 10.1089/vim.2022.0103) (PMID:36622942) (PMCID:PMC9885547)
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Abstract
Cache Valley virus (CVV) is a mosquito-borne bunyavirus that is enzootic throughout the new world. Although CVV is known as an important agricultural pathogen, primarily associated with embryonic lethality and abortions in ruminants, it has recently been recognized for its expansion as a zoonotic pathogen. With the increased emergence of bunyaviruses with human and veterinary importance, there have been significant efforts dedicated to the development of bunyavirus vaccines. In this study, the immunogenicity of a candidate live-attenuated vaccine (LAV) for CVV, which contains the deletion of the nonstructural small (NSs) and nonstructural medium (NSm) genes (2delCVV), was evaluated and compared with an autogenous candidate vaccine created through the inactivation of CVV using binary ethylenimine (BEI) with an aluminum hydroxide adjuvant (BEI-CVV) in sheep. Both 2delCVV and BEI-CVV produced a neutralizing antibody response that exceeds the correlate of protection, that is, plaque reduction neutralization test titer >10. However, on day 63 postinitial immunization, 2delCVV was more immunogenic than BEI-CVV. These results warrant further development of 2delCVV as a candidate LAV and demonstrate that the double deletion of the NSs and NSm genes can be applied to the development of vaccines and as a common attenuation strategy for orthobunyaviruses.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Dunlop, Dr James and Kohl, Professor Alain |
| Authors: | Ayers, V. B., Huang, Y.-J. S., Kohl, A., Dunlop, J. I., Hettenbach, S. M., Park, S. L., Higgs, S., and Vanlandingham, D. L. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Viral Immunology |
| Publisher: | Mary Ann Liebert |
| ISSN: | 0882-8245 |
| ISSN (Online): | 1557-8976 |
| Published Online: | 09 January 2023 |
| Copyright Holders: | Copyright © 2023 Victoria B. Ayers et al. |
| First Published: | First published in Viral Immunology 36(1): 41-47 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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