Serum anti‐GM2 and anti‐GalNAc‐GD1a ganglioside IgG antibodies are biomarkers for immune‐mediated polyneuropathies in cats

Halstead, S. K. et al. (2023) Serum anti‐GM2 and anti‐GalNAc‐GD1a ganglioside IgG antibodies are biomarkers for immune‐mediated polyneuropathies in cats. Journal of the Peripheral Nervous System, 28(1), pp. 32-40. (doi: 10.1111/jns.12529) (PMID:36573790)

Abstract

Background and Aims: Recent work identified anti-GM2 and anti-GalNAc-GD1a IgG ganglioside antibodies as biomarkers in dogs clinically diagnosed with acute canine polyradiculoneuritis, in turn considered a canine equivalent of Guillain-Barré syndrome. This study aims to investigate the serum prevalence of similar antibodies in cats clinically diagnosed with immune-mediated polyneuropathies. Methods: The sera from 41 cats clinically diagnosed with immune-mediated polyneuropathies (IPN), 9 cats with other neurological or neuromuscular disorders (ONM) and 46 neurologically normal cats (CTRL) were examined for the presence of IgG antibodies against glycolipids GM1, GM2, GD1a, GD1b, GalNAc-GD1a, GA1, SGPG, LM1, galactocerebroside and sulphatide. Results: A total of 29/41 IPN-cats had either anti-GM2 or anti-GalNAc-GD1a IgG antibodies, with 24/29 cats having both. Direct comparison of anti-GM2 (sensitivity: 70.7%; specificity: 78.2%) and anti-GalNAc-GD1a (sensitivity: 70.7%; specificity: 70.9%) antibodies narrowly showed anti-GM2 IgG antibodies to be the better marker for identifying IPN-cats when compared to the combined ONM and CTRL groups (p=0.049). Anti-GA1 and/or anti-sulphatide IgG antibodies were ubiquitously present across all sample groups, whereas antibodies against GM1, GD1a, GD1b, SGPG, LM1 and galactocerebroside were overall only rarely observed. Interpretation: Anti-GM2 and anti-GalNAc-GD1a IgG antibodies may serve as serum biomarkers for immune-mediated polyneuropathies in cats, as previously observed in dogs and humans.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Halstead, Dr Susan and Willison, Professor Hugh and Gutierrez Quintana, Mr Rodrigo and Kaczmarska, Mrs Adriana and Freeman, Mr Paul and Jose-Lopez, Mr Roberto and Jackson, Dr Mark and Rupp, Dr Angie
• Authors: Halstead, S. K., Jackson, M., Bianchi, E., Rupp, S., Granger, N., Menchetti, M., Galli, G., Freeman, P., Kaczmarska, A., Bhatti, S. F.M., Brocal, J., José‐López, R., Tipold, A., Gutierrez Quintana, R., Ives, E. J., Liatis, T., Nessler, J., Rusbridge, C., Willison, H. J., and Rupp, A.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine; College of Medical Veterinary and Life Sciences > School of Cancer Sciences; College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Research Centre: College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology
• Journal Name: Journal of the Peripheral Nervous System
• Publisher: Wiley
• ISSN: 1085-9489
• ISSN (Online): 1529-8027
• Published Online: 27 December 2022
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in Journal of the Peripheral Nervous System 28(1): 32-40
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
165079		The structural and functional diversity of anti-glycolipid antibody repertoires and their nerve binding domains in human autoimmune neuropathy	Hugh Willison	Wellcome Trust (WELLCOTR)	092805/Z/10/Z	III - Immunology
173549		Pathophysiological factors in the diagnosis and treatment of the Guillain-Barre syndromes	Hugh Willison	Wellcome Trust (WELLCOTR)	202789/Z/16/Z	III - Immunology
310863		Serum anti-glycolipid antibodies in cats suffering from peripheral neuropathies	Angelika Frances Rupp	Petplan Charitable Trust (PETPLAN)	S20-922-961	Vets - Veterinary Pathology, Public Health & Disease Investigation