Association of high immunohistochemical expression of minichromosome maintenance 3 with human oral squamous cell carcinoma—a preliminary study

Zahir, R. et al. (2023) Association of high immunohistochemical expression of minichromosome maintenance 3 with human oral squamous cell carcinoma—a preliminary study. Diagnostics, 13(1), 61. (doi: 10.3390/diagnostics13010061) (PMID:36611359) (PMCID:PMC9818330)

Abstract

Background: Oral squamous cell carcinoma (OSCC) may arise from premalignant oral lesions (PMOL) in most cases. Minichromosome maintenance 3 (MCM3) is a proliferative marker that has been investigated as a potential diagnostic biomarker in the diagnosis of oral cancer. Objectives: To evaluate the association of MCM3 expression, its clinicopathologic parameters and to identify snuff (also called naswar) as a potential risk factor for changes in MCM3 expression in PMOL and OSCC. Methodology: Immunohistochemistry (IHC) of MCM3 was performed on 32 PMOL, 32 OSCC and 16 normal controls after optimization of IHC methodology. Histoscore (0–300) was used as a scoring system and seven different cut-offs were identified for analyses. Data were analyzed using various statistical tests. Results: Among the seven cutoffs, 40% strong positive cells were found to be a better cut-off as they were associated with many pathological variables (Broder’s grade, Aneroth’s grade, and mitotic activity). The differential MCM3 expression in oral lesions (PMOL and OSCC) was statistically significant (p = 0.03). Moreover, MCM3 expression is raised with increased duration and frequency of snuff use. Conclusion: High MCM3 expression is associated with disease progression and is a potential indicator of malignant transformations from PMOL to OSCC. Moreover, the use of snuff is associated with MCM3 over-expression.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Ali, Dr Asif

Creator Roles

Ali, A.: Conceptualization, Formal analysis, Writing – review and editing

• Authors: Zahir, R., Khan, Z. A., Aleem, B., Ahmad, S., Ali, A., Issrani, R., Alruwaili, M. K., Iqbal, S., Alghumaiz, S. F., Alanazi, S. H., Umer, M. F., Ullah, I., Sumera, , and Ganji, K. K.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Diagnostics
• Publisher: MDPI
• ISSN: 2075-4418
• ISSN (Online): 2075-4418
• Published Online: 26 December 2022
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in Diagnostics 13(1): 61
• Publisher Policy: Reproduced under a Creative Commons License