Fluorous-directed assembly of DNA origami nanostructures

Zou, J. et al. (2023) Fluorous-directed assembly of DNA origami nanostructures. ACS Nano, 17(1), pp. 752-759. (doi: 10.1021/acsnano.2c10727) (PMID:36537902) (PMCID:PMC9835977)

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An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson–Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated “catch-and-latch” approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences. As a result, our integration of fluorous-directed assembly into origami systems represents a cheap, atom-efficient means to produce discrete superstructures.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Santana Vega, Dr Marina and Peveler, Dr William and Clark, Professor Alasdair and Withers, Dr Jamie and Christie, Mr Iain and Stammers, Dr Ashley and Zou, Jiajia and Aekbote, Dr Badri and Burley, Dr Glenn
Authors: Zou, J., Stammers, A. C., Taladriz-Sender, A., Withers, J. M., Christie, I., Santana Vega, M., Aekbote, B. L., Peveler, W. J., Rusling, D. A., Burley, G. A., and Clark, A. W.
College/School:College of Science and Engineering > School of Chemistry
College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:ACS Nano
Publisher:American Chemical Society
ISSN (Online):1936-086X
Published Online:20 December 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in ACS Nano 17(1): 752-759
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.1353

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
301408Macromolecular construction of nucleic acid networks directed by the flourous effect: A new paradigm for the programmable assembly of molecular informationAlasdair ClarkLeverhulme Trust (LEVERHUL)RPG-2018-149/ 170977ENG - Biomedical Engineering
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309846Decentralised water technologiesWilliam SloanEngineering and Physical Sciences Research Council (EPSRC)EP/V030515/1ENG - Infrastructure & Environment