Hu, C. , Annese, V. F. , Barrett, M. P. and Cumming, D. R.S. (2023) Point-of-care platform for diagnosis of venous thrombosis by simultaneous detection of thrombin generation and D-dimer in human plasma. Analytical Chemistry, 95(2), pp. 1115-1122. (doi: 10.1021/acs.analchem.2c03819) (PMID:36544272) (PMCID:PMC9850404)
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Abstract
Venous thromboembolism (VTE) refers to a blood clot that starts in a vein. The risk of developing VTE is highest after major surgery or a major injury, or when someone has heart failure, cancer, or infectious disease (e.g., COVID-19). Without prompt treatment to break up clots and prevent more from forming, VTE can restrict or block blood flow and oxygen, which can damage the body tissue or organs. VTE can occur without any obvious signs, and imaging technologies are used. Alternatively rapid measurement of thrombin generation (TG) and D-dimer could be used to make a fast, portable, and easy-to-use diagnostic platform for VTE. Here, we have demonstrated a diagnostic sensing platform with the ability of simultaneous detection of TG and D-dimer in human plasma. Modifications were made to both the assay protocols to eliminate the need for sample dilution and incubation steps. Using a substantially reduced sample volume, the measurement results show comparable performance to the gold standard method. Our platform is able to deliver accurate and cost-effective results for both TG and D-dimer assays when using undiluted plasma in under 15 min. The assays presented are therefore a good candidate technology for use in a point-of-care platform to diagnose VTE.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cumming, Professor David and Hu, Dr Chunxiao and Annese, Dr Valerio and Barrett, Professor Michael |
| Authors: | Hu, C., Annese, V. F., Barrett, M. P., and Cumming, D. R.S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Science and Engineering > School of Engineering > Biomedical Engineering College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering |
| Journal Name: | Analytical Chemistry |
| Publisher: | American Chemical Society |
| ISSN: | 0003-2700 |
| ISSN (Online): | 1520-6882 |
| Published Online: | 21 December 2022 |
| Copyright Holders: | Copyright © 2022 The Authors |
| First Published: | First published in Analytical Chemistry 95(2): 1115-1122 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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