Brown, J. L. , Butcher, M. C., Ramalingam Veena, C. L., Chogule, S., Johnston, W. and Ramage, G. (2023) Generation of multispecies oral bacteria biofilm models. In: Seymour, G. J., Cullinan, M. P., Heng, N. C. K. and Cooper, P. R. (eds.) Oral Biology: Molecular Techniques and Applications. Series: Methods in Molecular Biology, 2588. Humana Press: New York, pp. 187-199. ISBN 9781071627792 (doi: 10.1007/978-1-0716-2780-8_12)
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Abstract
It is well-recognized that oral biofilms that occur in health and disease have a polymicrobial composition, though these are poorly reflected in the literature, with many studies focussing on simple mono-species biofilm model systems. The utility of polymicrobial biofilm model systems is that they more accurately reflect the oral cavity and allow researchers to ask relevant questions in basic science studies, pharmaceutical screening, and investigating inflammatory interactions. Here we describe the detailed methodology of how to sequentially construct and maintain polymicrobial biofilm models pertinent to caries, periodontal disease, and denture stomatitis.
Item Type: | Book Sections |
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Status: | Published |
Glasgow Author(s) Enlighten ID: | Butcher, Mr Mark and Ramage, Professor Gordon and Chogule, Dr Safa and Johnston, William and Ramalingam Veena, Dr Chandra Lekha and Brown, Dr Jason |
Authors: | Brown, J. L., Butcher, M. C., Ramalingam Veena, C. L., Chogule, S., Johnston, W., and Ramage, G. |
College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School |
Publisher: | Humana Press |
ISSN: | 1064-3745 |
ISBN: | 9781071627792 |
Published Online: | 24 November 2022 |
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