Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse

Paterson, S., Holmes, W. and Rodgers, J. (2022) Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse. PLoS Neglected Tropical Diseases, 16(12), e0010962. (doi: 10.1371/journal.pntd.0010962) (PMID:36477669) (PMCID:PMC9728833)

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Splenomegaly, an enlargement of the spleen, is a known clinical sign of the parasitic disease, human African trypanosomiasis. This study follows the development of splenomegaly in a group of mice over multiple infection points, using a non-invasive imaging modality, magnetic resonance imaging (MRI). CD-1 mice infected with GVR35 T.b. brucei demonstrated a significant increase in spleen size from day 7 post-infection, with changes in the spleen tracked in individual animals over five time points. At the final time point, the mean spleen weight calculated using the spleen volume from the MR images was compared with the post-mortem gross spleen weight. No significant difference was detected between the two methods (1.62 ± 0.06g using MRI and 1.51 ± 0.04g gross weight, p = 0.554). Haematology and histological analysis were also performed, giving additional insight into splenomegaly for the GVR35 strain of infection. The study demonstrates that MRI is a useful tool when examining changes in organ volume throughout HAT infection and may be applicable in the investigation of a range of conditions where changes in organ volume occur and MRI has not been used previously.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Rodgers, Dr Jean and Holmes, Dr William
Authors: Paterson, S., Holmes, W., and Rodgers, J.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
Journal Name:PLoS Neglected Tropical Diseases
Publisher:Public Library of Science
ISSN (Online):1935-2735
Copyright Holders:Copyright © 2022 Paterson et al
First Published:First published in PLoS Neglected Tropical Diseases 16(12):e0010962
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190906EPSRC 2015 DTPMary Beth KneafseyEngineering and Physical Sciences Research Council (EPSRC)EP/M508056/1Research and Innovation Services