Hwang, J. Y., Lim, H. G., Yoon, C. W., Lam, K. H. , Yoon, S., Lee, C., Chiu, C. T., Kang, B. J., Kim, H. H. and Shung, K. K. (2014) Non-contact high-frequency ultrasound microbeam stimulation for studying mechanotransduction in human umbilical vein endothelial cells. Ultrasound in Medicine and Biology, 40(9), pp. 2172-2182. (doi: 10.1016/j.ultrasmedbio.2014.03.018) (PMID:25023109) (PMCID:PMC4130794)
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Abstract
We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Lam, Dr Koko |
| Authors: | Hwang, J. Y., Lim, H. G., Yoon, C. W., Lam, K. H., Yoon, S., Lee, C., Chiu, C. T., Kang, B. J., Kim, H. H., and Shung, K. K. |
| College/School: | College of Science and Engineering > School of Engineering > Systems Power and Energy |
| Journal Name: | Ultrasound in Medicine and Biology |
| Publisher: | Elsevier |
| ISSN: | 0301-5629 |
| ISSN (Online): | 1879-291X |
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