Revealing stromal and lymphoid sources of Col3a1-expression during inflammation using a novel reporter mouse

Camargo da Rosa, L. , Scales, H. E. , Makhija, S., Sutherland, K., Benson, R. A. , Brewer, J. M. and Garside, P. (2022) Revealing stromal and lymphoid sources of Col3a1-expression during inflammation using a novel reporter mouse. Discovery Immunology, 1(1), kyac008. (doi: 10.1093/discim/kyac008)

Abstract

One of the earliest signs of dysregulation of the homeostatic process of fibrosis, associated with pathology in chronic conditions such as rheumatoid arthritis (RA), is the overexpression of collagen type III (COL-3). Critically, there is still relatively little known regarding the identity of the cell types expressing the gene encoding COL-3 (Col3a1). Identifying and characterising Col3a1-expressing cells during the development of fibrosis could reveal new targets for diagnosis and treatment of fibrosis-related pathologies. As such, a reporter mouse expressing concomitantly Col3a1 and mKate-2, a fluorescent protein, was generated. Using models of footpad inflammation, we demonstrated its effectiveness as a tool to measure the expression of COL-3 during the repair process and provided an initial characterisation of some of the stromal and immune cells responsible for Col3a1 expression.

• Item Type: Articles
• Additional Information: This research received support from a variety of sources at University of Glasgow.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Benson, Dr Robert and Garside, Professor Paul and Brewer, Professor James and Camargo da Rosa, Dr Larissa and Scales, Dr Hannah
• Authors: Camargo da Rosa, L., Scales, H. E., Makhija, S., Sutherland, K., Benson, R. A., Brewer, J. M., and Garside, P.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Research Centre: College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology
• Journal Name: Discovery Immunology
• Publisher: Oxford University Press
• ISSN: 2754-2483
• ISSN (Online): 2754-2483
• Published Online: 21 November 2022
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in Discovery Immunology 1(1): kyac008
• Publisher Policy: Reproduced under a Creative Commons License