Proteomic changes associated with maternal dietary low ω6:ω3 ratio in piglets supplemented with seaweed part II: ileum proteomes

Nguyen, T. X., Agazzo, A., McGill, S., Weidt, S., Han, Q. H., Gelemanović, A., McLaughlin, M. , Savoini, G., Eckersall, P. D. and Burchmore, R. (2023) Proteomic changes associated with maternal dietary low ω6:ω3 ratio in piglets supplemented with seaweed part II: ileum proteomes. Journal of Proteomics, 270, 104739. (doi: 10.1016/j.jprot.2022.104739) (PMID:36174954)

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Abstract

This study evaluates how long-term dietary low ω6:ω3 ratio in sows and offspring's seaweed (SW) intake affects piglet intestinal function and growth through modifying ileum proteome. Sows were assigned to either control diet (CR, ω6:ω3 ratio = 13:1) or treatment diet (LR, ω6:ω3 = 4:1) during gestation and lactation (n = 8 each). The male weaned offspring were received a basal diet with or without SW powder supplementation (4 g/kg) for 21 days, denoted as SW and CT groups, respectively. In total, four groups of weaned piglets were formed following maternal and offspring's diets combination, represented by CRCT, CRSW, LRCT, and LRSW (n = 10 each). Piglet ileum tissue was collected on day 22 post-weaning and analysed using TMT-based quantitative proteomics. The differentially abundant proteins (n = 300) showed the influence of maternal LR diet on protein synthesis, cell proliferation, and cell cycle regulation. In contrast, the SW diet lowered the inflammation severity and promoted ileal tissue development in CRSW piglets but reduced the fat absorption capacity in LRSW piglets. These results uncovered the mechanism behind the anti-inflammation and intestinal-boosting effects of maternal LR diet in piglets supplemented with SW.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McLaughlin, Dr Mark and McGill, Mrs Suzanne and Burchmore, Dr Richard and Eckersall, Professor David and Weidt, Dr Stefan and Nguyen, Thi Xuan
Authors: Nguyen, T. X., Agazzo, A., McGill, S., Weidt, S., Han, Q. H., Gelemanović, A., McLaughlin, M., Savoini, G., Eckersall, P. D., and Burchmore, R.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
College of Medical Veterinary and Life Sciences > School of Cancer Sciences
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of Proteomics
Publisher:Elsevier
ISSN:1874-3919
ISSN (Online):1876-7737
Published Online:27 September 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Journal of Proteomics 270: 104739
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
303918Joint Doctorate in Molecular Animal NutritionPeter EckersallEuropean Commission (EC)765423Institute of Biodiversity, Animal Health and Comparative Medicine