Chan, Y., Patterson, C., Priest, J. W., Stresman, G., William, T., Chua, T. H., Tetteh, K. K., Lammie, P., Drakeley, C. and Fornace, K. M. (2022) Assessing seroprevalence and associated risk factors for multiple infectious diseases in Sabah, Malaysia using serological multiplex bead assays. Frontiers in Public Health, 10, 924316. (doi: 10.3389/fpubh.2022.924316) (PMID:36388287) (PMCID:PMC9641279)
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Abstract
Background: Infectious diseases continue to burden populations in Malaysia, especially among rural communities where resources are limited and access to health care is difficult. Current epidemiological trends of several neglected tropical diseases in these populations are at present absent due to the lack of habitual and efficient surveillance. To date, various studies have explored the utility of serological multiplex beads to monitor numerous diseases simultaneously. We therefore applied this platform to assess population level exposure to six infectious diseases in Sabah, Malaysia. Furthermore, we concurrently investigated demographic and spatial risk factors that may be associated with exposure for each disease. Methods: This study was conducted in four districts of Northern Sabah in Malaysian Borneo, using an environmentally stratified, population-based cross-sectional serological survey targeted to determine risk factors for malaria. Samples were collected between September to December 2015, from 919 villages totaling 10,100 persons. IgG responses to twelve antigens of six diseases (lymphatic filariasis- Bm33, Bm14, BmR1, Wb123; strongyloides- NIE; toxoplasmosis-SAG2A; yaws- Rp17 and TmpA; trachoma- Pgp3, Ct694; and giardiasis- VSP3, VSP5) were measured using serological multiplex bead assays. Eight demographic risk factors and twelve environmental covariates were included in this study to better understand transmission in this community. Results: Seroprevalence of LF antigens included Bm33 (10.9%), Bm14+ BmR1 (3.5%), and Wb123 (1.7%). Seroprevalence of Strongyloides antigen NIE was 16.8%, for Toxoplasma antigen SAG2A was 29.9%, and Giardia antigens GVSP3 + GVSP5 was 23.2%. Seroprevalence estimates for yaws Rp17 was 4.91%, for TmpA was 4.81%, and for combined seropositivity to both antigens was 1.2%. Seroprevalence estimates for trachoma Pgp3 + Ct694 were 4.5%. Age was a significant risk factors consistent among all antigens assessed, while other risk factors varied among the different antigens. Spatial heterogeneity of seroprevalence was observed more prominently in lymphatic filariasis and toxoplasmosis. Conclusions: Multiplex bead assays can be used to assess serological responses to numerous pathogens simultaneously to support infectious disease surveillance in rural communities, especially where prevalences estimates are lacking for neglected tropical diseases. Demographic and spatial data collected alongside serosurveys can prove useful in identifying risk factors associated with exposure and geographic distribution of transmission.
| Item Type: | Articles |
|---|---|
| Additional Information: | We acknowledge the UK Medical Research Council, Natural Environment Research Council, Economic and Social Research Council and Biotechnology and Biosciences Research Council for the funding received for this project through the Environmental and Social Ecology of Human Infectious Diseases Initiative (grant number G1100796). KMF is supported by a Sir Henry Dale fellowship jointly funded by the Wellcome Trust and Royal Society (grant number 221963/Z/20/Z). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Fornace, Dr Kimberly |
| Authors: | Chan, Y., Patterson, C., Priest, J. W., Stresman, G., William, T., Chua, T. H., Tetteh, K. K., Lammie, P., Drakeley, C., and Fornace, K. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Frontiers in Public Health |
| Publisher: | Frontiers Media |
| ISSN: | 2296-2565 |
| ISSN (Online): | 2296-2565 |
| Published Online: | 25 October 2022 |
| Copyright Holders: | Copyright © 2022 Chan, Patterson, Priest, Stresman, William, Chua, Tetteh, Lammie, Drakeley and Fornace |
| First Published: | First published in Frontiers in Public Health 10: 924316 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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