Felion, C., Lopez-Gonzalez, R., Sewell, A. L., Marquez, R. and Gauchotte-Lindsay, C. (2022) BODIPY-labeled estrogens for fluorescence analysis of environmental microbial degradation. ACS Omega, 7(45), pp. 41284-41295. (doi: 10.1021/acsomega.2c05002) (PMID:36406552) (PMCID:PMC9670910)
![[img]](https://eprints.gla.ac.uk/style/images/fileicons/text.png) |
Text
281749.pdf - Published Version Available under License Creative Commons Attribution. 4MB |
|
Text
281749Suppl1.pdf - Supplemental Material 1MB |
Abstract
Biodegradation of estrogen hormone micropollutants is a well-established approach toward their remediation. Fluorescently labeled substrates are used extensively for rapid, near-real-time analysis of biological processes and are a potential tool for studying biodegradation processes faster and more efficiently than conventional approaches. However, it is important to understand how the fluorescently tagged surrogates compare with the natural substrate in terms of chemical analysis and the intended application. We derivatized three natural estrogens with BODIPY fluorophores by azide–alkyne cycloaddition click reaction and developed an analytical workflow based on simple liquid–liquid extraction and HPLC-PDA analysis. The developed methods allow for concurrent analysis of both fluorescent and natural estrogens with comparable recovery, accuracy, and precision. We then evaluated the use of BODIPY-labeled estrogens as surrogate substrates for studying biodegradation using a model bacterium for estrogen metabolism. The developed analytical methods were successfully employed to compare the biological transformation of 17β-estradiol (E2), with and without the BODIPY fluorescent tag. Through measuring the complete degradation of E2 and the transformation of BODIPY-estradiol to BODIPY-estrone in the presence of a co-substrate, we found that BODIPY-labeled estrogens are biologically viable surrogates for investigating biodegradation in environmental bacteria.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Sewell, Mr Alan and Felion, Ms Celeste Marie and Gauchotte-Lindsay, Professor Caroline |
| Authors: | Felion, C., Lopez-Gonzalez, R., Sewell, A. L., Marquez, R., and Gauchotte-Lindsay, C. |
| College/School: | College of Science and Engineering > School of Chemistry College of Science and Engineering > School of Engineering > Infrastructure and Environment |
| Journal Name: | ACS Omega |
| Publisher: | American Chemical Society |
| ISSN: | 2470-1343 |
| ISSN (Online): | 2470-1343 |
| Published Online: | 03 November 2022 |
| Copyright Holders: | Copyright © 2022 The Authors |
| First Published: | First published in ACS Omega 7(45): 41284-41295 |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information