Martins, C. F. et al. (2022) Effect of dietary Spirulina (Arthrospira platensis) on the intestinal function of post-weaned piglet: An approach combining proteomics, metabolomics and histological studies. Journal of Proteomics, 269, 104726. (doi: 10.1016/j.jprot.2022.104726) (PMID:36096433)
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Abstract
The effect of dietary Spirulina (Arthrospira platensis) and CAZyme supplementation was assessed on the gut of weaned piglets, using an integrated NMR-metabolomics approach combined with Tandem Mass Tag labelled proteomics. Thirty weaned male piglets were assigned to one of the three following diets (n = 10): cereal and soybean meal basal diet (Control), basal diet with 10% Spirulina inclusion (SP) and SP diet supplemented with 0.01% lysozyme (SP + L). The experiment lasted 4 weeks and, upon slaughter, small intestine samples were collected for histological, metabolomic and proteomic analysis. No significant differences were found for the histology and metabolomics analysis between the three experimental groups. Lactate, glutamate, glycine and myo-inositol were the most abundant metabolites. Proteomics results showed 1502 proteins identified in the intestine tissue. A total of 23, 78, 27 differentially abundant proteins were detected respectively for the SP vs. Control, SP + L vs. Control and SP + L vs. SP comparisons. The incorporation of Spirulina and supplementation of lysozyme in the piglet's diets is associated to intestinal proteomic changes. These include increased protein synthesis and abundance of contractile apparatus proteins, related with increased nutrient availability, which has beneficial (increased glucose uptake) and detrimental (increased digesta viscosity) metabolic effects.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by Fundaçao ˜ para a Ciˆencia e a Tecnologia (FCT, Lisbon, Portugal) grant (PTDC/CVT-NUT/5931/2014), Portugal2020 grant (08/SI/3399/2015), CIISA research center (UIDB/ 00276/2020) and LEAF research center (UIDB/04129/2020) and European Commission (FP7 “VetMedZg” Project, grant number 621394). Author DMR acknowledges a PhD fellowship (SFRH/BD/143992/2019) also granted by FCT. |
| Keywords: | Metabolomics, Spirulina, Piglets, Intestine, Proteomics |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Eckersall, Professor David |
| Authors: | Martins, C. F., Ribeiro, D. M., Matzapetakis, M., Pinho, M. A., Kuleš, J., Horvatić, A., Guillemin, N., Eckersall, P. D., Freire, J. P. B., de Almeida, A. M., and Prates, J. A. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Journal of Proteomics |
| Publisher: | Elsevier |
| ISSN: | 1874-3919 |
| ISSN (Online): | 1876-7737 |
| Published Online: | 10 September 2022 |
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