Lyngby, J. G. et al. (2022) Association of fecal and serum microRNA profiles with gastrointestinal cancer and chronic inflammatory enteropathy in dogs. Journal of Veterinary Internal Medicine, 36(6), pp. 1989-2001. (doi: 10.1111/jvim.16530) (PMID:36120988)
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Abstract
Background: Reliable biomarkers to differentiate gastrointestinal cancer (GIC) from chronic inflammatory enteropathy (CIE) in dogs are needed. Fecal and serum microRNAs (miRNAs) have been proposed as diagnostic and prognostic markers of GI disease in humans and dogs. Hypothesis/Objectives: Dogs with GIC have fecal and serum miRNA profiles that differ from those of dogs with CIE. Aims: (a) identify miRNAs that differentiate GIC from CIE, (b) use high‐throughput reverse transcription quantitative real‐time PCR (RT‐qPCR) to establish fecal and serum miRNA panels to distinguish GIC from CIE in dogs. Animals: Twenty‐four dogs with GIC, 10 dogs with CIE, and 10 healthy dogs, all client‐owned. Methods: An international multicenter observational prospective case‐control study. Small RNA sequencing was used to identify fecal and serum miRNAs, and RT‐qPCR was used to establish fecal and serum miRNA panels with the potential to distinguish GIC from CIE. Results: The best diagnostic performance for distinguishing GIC from CIE was fecal miR‐451 (AUC: 0.955, sensitivity: 86.4%, specificity: 100%), miR‐223 (AUC: 0.918, sensitivity: 90.9%, specificity: 80%), and miR‐27a (AUC: 0.868, sensitivity: 81.8%, specificity: 90%) and serum miR‐20b (AUC: 0.905, sensitivity: 90.5%, specificity: 90%), miR‐148a‐3p (AUC: 0.924, sensitivity: 85.7%, specificity: 90%), and miR‐652 (AUC: 0.943, sensitivity: 90.5%, specificity: 90%). Slightly improved diagnostic performance was achieved when combining fecal miR‐451 and miR‐223 (AUC: 0.973, sensitivity: 95.5%, specificity: 90%). Conclusions and Clinical Importance: When used as part of a diagnostic RT‐qPCR panel, the abovementioned miRNAs have the potential to function as noninvasive biomarkers for the differentiation of GIC and CIE in dogs.
Item Type: | Articles |
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Keywords: | Biomarker, CIE, miRNA qPCR, neoplasia, RT‐qPCR, small RNA sequencing. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Morris, Professor Joanna |
Authors: | Lyngby, J. G., Gòdia, M., Brogaard, L., Kristensen, A. T., Fredholm, M., Skancke, E., Morris, J., Dupont, N., Salavati Schmitz, S., Argyle, D., Sánchez, A., Bjørnvad, C. R., Cirera, S., and Nielsen, L. N. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Journal of Veterinary Internal Medicine |
Publisher: | Wiley |
ISSN: | 0891-6640 |
ISSN (Online): | 1939-1676 |
Published Online: | 19 September 2022 |
Copyright Holders: | Copyright © 2022 The Authors |
First Published: | First published in Journal of Veterinary Internal Medicine 36(6): 1989-2001 |
Publisher Policy: | Reproduced under a Creative Commons licence |
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