Tezcan, N., Özdemir-Kumral, Z. N., Özkan Yenal, N., Çilingir-Kaya, Ö. T., Virlan, A. T. , Özbeyli, D., Çetinel, Ş., Yeğen, B. Ç. and Koç, M. (2022) Nesfatin-1 treatment preserves antioxidant status and attenuates renal fibrosis in rats with unilateral ureteral obstruction. Nephrology Dialysis Transplantation, 37(7), pp. 1238-1248. (doi: 10.1093/ndt/gfac053) (PMID:35218196)
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Abstract
Background: Nesfatin-1 (NES-1), an anorexigenic peptide, was reported to have anti-inflammatory and anti-apoptotic actions in several inflammation models. Methods: To elucidate potential renoprotective effects of NES-1, unilateral ureteral obstruction (UUO) was induced in male Sprague Dawley rats by ligating left ureters. The rats were injected intraperitoneally with either saline (SL) or NES-1 (10 µg/kg/day) for 7 or 14 days (n = 8 in each group). On the 7th or 14th day, obstructed kidneys were removed for the isolation of leucocytes for flow-cytometric analysis and the assessments of biochemical and histopathological changes. Results: Opposite to glutathione levels, renal myeloperoxidase activity in the SL-treated UUO group was significantly increased compared with the sham-operated group, while NES-1 treatment abolished the elevation. The percentages of CD8+/CD4+ T-lymphocytes infiltrating the obstructed kidneys were increased in the SL-treated groups but treatment with NES-1 did not prevent lymphocyte infiltration. Elevated tumour necrosis factor-alpha (TNF-α) levels in SL-treated UUO group were decreased with NES-1. Although total degeneration scores were similarly increased in all UUO groups, tubular dilatation scores were significantly increased in UUO groups and lowered by NES-1 only in the 7-day treated group. Elevated interstitial fibrosis scores in the SL-treated groups were decreased in both 7- and 14-day NES-1 treated groups, while alpha-smooth muscle actin (α-SMA) and apoptosis scores were depressed in both NES-1 treated groups. Conclusion: The present data demonstrate that UUO-induced renal fibrosis is ameliorated by NES-1, which appears to involve the inhibition of neutrophil infiltration and thereby amelioration of oxidative stress and inflammation. These data suggest that NES-1 may have a regulatory role in protecting the kidneys against obstruction-induced renal injury.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Tulunay Virlan, Dr Aysin |
| Authors: | Tezcan, N., Özdemir-Kumral, Z. N., Özkan Yenal, N., Çilingir-Kaya, Ö. T., Virlan, A. T., Özbeyli, D., Çetinel, Ş., Yeğen, B. Ç., and Koç, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Nephrology Dialysis Transplantation |
| Publisher: | Oxford University Press |
| ISSN: | 0931-0509 |
| ISSN (Online): | 1460-2385 |
| Published Online: | 26 February 2022 |
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