Microarray analysis of the in vivo response of microglia to Aβ peptides in mice with conditional deletion of the prostaglandin EP2 receptor

Johansson, J.U., Woodling, N.S. , Brown, H.D., Wang, Q. and Andreasson, K.I. (2015) Microarray analysis of the in vivo response of microglia to Aβ peptides in mice with conditional deletion of the prostaglandin EP2 receptor. Genomics Data, 5, pp. 268-271. (doi: 10.1016/j.gdata.2015.06.011) (PMID:26251825) (PMCID:PMC4522932)

Abstract

Amyloid-β (Aβ) peptides accumulate in the brains of patients with Alzheimer's disease (AD), where they generate a persistent inflammatory response from microglia, the innate immune cells of the brain. The immune modulatory cyclooxygenase/prostaglandin E2 (COX/PGE2) pathway has been implicated in preclinical AD development, both in human epidemiology studies and in transgenic rodent models of AD [2,3]. PGE2 signals through four G-protein-coupled receptors, including the EP2 receptor that has been investigated for its role in mediating the inflammatory and phagocytic responses to Aβ [4]. To identify transcriptional differences in microglia lacking the EP2 receptor, we examined mice with EP2 conditionally deleted in Cd11b-expressing immune cells. We injected Aβ peptides or saline vehicle into the brains of adult mice, isolated primary microglia, and analyzed RNA expression by microarray. The resulting datasets were analyzed in two studies [5,6], one describing the basal status of microglia with or without EP2 deletion, and the second study analyzing the microglial response to Aβ. Here we describe in detail the experimental design and data analyses. The raw data from these studies are deposited in GEO, accession GSE57181 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57181).

• Item Type: Articles
• Additional Information: This work was supported by NIH grant RO1AG030209 (to K.I. Andreasson), NIH grant R21AG033914 (to K.I. Andreasson), Alzheimer' Association (IIRG-06-27502) (to K.I. Andreasson), Swedish Research Council (to J.U. Johansson), National Science Foundation (to N.S. Woodling), and NRSA grant F31AG039195 (to N.S. Woodling).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Woodling, Dr Nathan
• Authors: Johansson, J.U., Woodling, N.S., Brown, H.D., Wang, Q., and Andreasson, K.I.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Genomics Data
• Publisher: Elsevier
• ISSN: 2213-5960
• Published Online: 14 June 2015
• Copyright Holders: Copyright © 2015 The Authors
• First Published: First published in Genomics Data 5:268-271
• Publisher Policy: Reproduced under a Creative Commons licence