Independent glial subtypes delay development and extend healthy lifespan upon reduced insulin-PI3K signalling

Woodling, N. S. , Rajasingam, A., Minkley, L. J., Rizzo, A. and Partridge, L. (2020) Independent glial subtypes delay development and extend healthy lifespan upon reduced insulin-PI3K signalling. BMC Biology, 18(1), 124. (doi: 10.1186/s12915-020-00854-9) (PMID:32928209) (PMCID:PMC7490873)

Abstract

Background The increasing age of global populations highlights the urgent need to understand the biological underpinnings of ageing. To this end, inhibition of the insulin/insulin-like signalling (IIS) pathway can extend healthy lifespan in diverse animal species, but with trade-offs including delayed development. It is possible that distinct cell types underlie effects on development and ageing; cell-type-specific strategies could therefore potentially avoid negative trade-offs when targeting diseases of ageing, including prevalent neurodegenerative diseases. The highly conserved diversity of neuronal and non-neuronal (glial) cell types in the Drosophila nervous system makes it an attractive system to address this possibility. We have thus investigated whether IIS in distinct glial cell populations differentially modulates development and lifespan in Drosophila. Results We report here that glia-specific IIS inhibition, using several genetic means, delays development while extending healthy lifespan. The effects on lifespan can be recapitulated by adult-onset IIS inhibition, whereas developmental IIS inhibition is dispensable for modulation of lifespan. Notably, the effects we observe on both lifespan and development act through the PI3K branch of the IIS pathway and are dependent on the transcription factor FOXO. Finally, IIS inhibition in several glial subtypes can delay development without extending lifespan, whereas the same manipulations in astrocyte-like glia alone are sufficient to extend lifespan without altering developmental timing. Conclusions These findings reveal a role for distinct glial subpopulations in the organism-wide modulation of development and lifespan, with IIS in astrocyte-like glia contributing to lifespan modulation but not to developmental timing. Our results enable a more complete picture of the cell-type-specific effects of the IIS network, a pathway whose evolutionary conservation in humans make it tractable for therapeutic interventions. Our findings therefore underscore the necessity for cell-type-specific strategies to optimise interventions for the diseases of ageing.

• Item Type: Articles
• Additional Information: We gratefully acknowledge the Wellcome Trust (WT098565/Z/12/Z to L.P.), the Max Planck Society (L.P.), and Alzheimer’s Society (AS-JF-17b-011 to N.S.W.) for funding this work
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Woodling, Dr Nathan

Creator Roles

Woodling, N.: Conceptualization, Supervision, Project administration, Funding acquisition, Methodology, Investigation, Formal analysis, Visualization, Writing – original draft, Writing – review and editing

• Authors: Woodling, N. S., Rajasingam, A., Minkley, L. J., Rizzo, A., and Partridge, L.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: BMC Biology
• Publisher: BioMed Central
• ISSN: 1741-7007
• ISSN (Online): 1741-7007
• Copyright Holders: Copyright: © The Author(s). 2020
• First Published: First published in BMC Biology 18(1): 124
• Publisher Policy: Reproduced under a Creative Commons licence

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