Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles

Haney, J., Vijayakrishnan, S. , Streetley, J. , Dee, K., Goldfarb, D. M., Clarke, M. , Mullin, M., Carter, S. D. , Bhella, D. and Murcia, P. R. (2022) Coinfection by influenza A virus and respiratory syncytial virus produces hybrid virus particles. Nature Microbiology, 7(11), pp. 1879-1890. (doi: 10.1038/s41564-022-01242-5) (PMID:36280786)

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Abstract

Interactions between respiratory viruses during infection affect transmission dynamics and clinical outcomes. To identify and characterize virus–virus interactions at the cellular level, we coinfected human lung cells with influenza A virus (IAV) and respiratory syncytial virus (RSV). Super-resolution microscopy, live-cell imaging, scanning electron microscopy and cryo-electron tomography revealed extracellular and membrane-associated filamentous structures consistent with hybrid viral particles (HVPs). We found that HVPs harbour surface glycoproteins and ribonucleoproteins of IAV and RSV. HVPs use the RSV fusion glycoprotein to evade anti-IAV neutralizing antibodies and infect and spread among cells lacking IAV receptors. Finally, we show that IAV and RSV coinfection in primary cells of the bronchial epithelium results in viral proteins from both viruses co-localizing at the apical cell surface. Our observations define a previously unknown interaction between respiratory viruses that might affect virus pathogenesis by expanding virus tropism and enabling immune evasion.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Vijayakrishnan, Dr Swetha and Haney, Ms Joanne and Carter, Dr Stephen and Bhella, Professor David and Clarke, Dr Mairi and Goldfarb, Daniel Max and Mullin, Mrs Margaret and Murcia, Professor Pablo and Streetley, Dr James and Dee, Dr Kieran
Authors: Haney, J., Vijayakrishnan, S., Streetley, J., Dee, K., Goldfarb, D. M., Clarke, M., Mullin, M., Carter, S. D., Bhella, D., and Murcia, P. R.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Nature Microbiology
Publisher:Nature Research
ISSN:2058-5276
ISSN (Online):2058-5276
Published Online:24 October 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Nature Microbiology 7(11): 1879-1890
Publisher Policy:Reproduced in accordance with the publisher copyright policy
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172630002Respiratory Infections: Epidemiology, evolution and ecology of respiratory viruses (Programme 1)Pablo MurciaMedical Research Council (MRC)MC_UU_12014/9III - Centre for Virus Research
304008MRC Flexible SupplementMorven BarlassMedical Research Council (MRC)MR/N013166/1 - LGH/MS/MEDCAMS - Cardiovascular Science
313949National Productivity Investment Fund StudentshipsGeorge BaillieMedical Research Council (MRC)MR/R502327/1MVLS - Graduate School
172630012Virus Structure (Programme 8)David BhellaMedical Research Council (MRC)MC_UU_12014/7III - Centre for Virus Research
301445The Scottish Macromolecular Imaging Centre (SMIC)David BhellaMedical Research Council (MRC)MC_PC_17135III - Centre for Virus Research
301474The purchase and maintenance of a cryo-electron microscopeDavid BhellaScottish Funding Council (SFC)H17007III - Centre for Virus Research