Cell type-specific calcium imaging of central sensitization in mouse dorsal horn

Warwick, C., Salsovic, J., Hachisuka, J. , Smith, K. M., Sheahan, T. D., Chen, H., Ibinson, J., Koerber, H. R. and Ross, S. E. (2022) Cell type-specific calcium imaging of central sensitization in mouse dorsal horn. Nature Communications, 13, 5199. (doi: 10.1038/s41467-022-32608-2) (PMID:36057681) (PMCID:PMC9440908)

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Abstract

Allodynia is a state in which pain is elicited by innocuous stimuli. Capsaicin applied to the skin results in an allodynia that extends to a broad region beyond the application site. This sensitization is thought to be mediated by spinal networks; however, we do not have a clear picture of which spinal neurons mediate this phenomenon. To address this gap, we used two-photon calcium imaging of excitatory interneurons and spinal projection neurons in the mouse spinal dorsal horn. To distinguish among neuronal subtypes, we developed CICADA, a cell profiling approach to identify cell types during calcium imaging. We then identified capsaicin-responsive and capsaicin-sensitized neuronal populations. Capsaicin-sensitized neurons showed emergent responses to innocuous input and increased receptive field sizes consistent with psychophysical reports. Finally, we identified spinal output neurons that showed enhanced responses from innocuous input. These experiments provide a population-level view of central sensitization and a framework with which to model somatosensory integration in the dorsal horn.

Item Type:Articles
Additional Information:The research reported in this publication was supported by the National Institute of Neurological Disorder and Stroke of the National Institutes of Health under Award Number R01 NS096705 to H.R. Koerber, NS110155 to T.D. Sheahan, NS073548 to C.A. Warwick, and the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health under Award Number R01AR063772 to S.E. Ross.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hachisuka, Dr Junichi
Authors: Warwick, C., Salsovic, J., Hachisuka, J., Smith, K. M., Sheahan, T. D., Chen, H., Ibinson, J., Koerber, H. R., and Ross, S. E.
College/School:College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
Journal Name:Nature Communications
Publisher:Nature Research
ISSN:2041-1723
ISSN (Online):2041-1723
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Nature Communications 13: 5199
Publisher Policy:Reproduced under a Creative Commons License

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