Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis

Borsanelli, A. C., Athayde, F. R. F., Riggio, M. P. , Brandt, B. W., Rocha, F. I., Jesus, E. C., Gaetti-Jardim, E., Schweitzer, C. M. and Dutra, I. S. (2022) Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis. Frontiers in Microbiology, 13, 936021. (doi: 10.3389/fmicb.2022.936021) (PMID:36033883) (PMCID:PMC9412940)

Abstract

Extensive cattle livestock is advancing in Amazonia and its low productivity, with consequent pressure to open new areas, is partly due to sanitary problems and, among them, the periodontal diseases, whose environmental triggers or modifying factors are unknown. In this study, we used high-throughput sequencing, network analysis and predicted functions to investigate the dental and ruminal microbiota of cattle raised in new livestock areas in the Amazon and identify possible keystone pathogens and proteins associated with the disease. Ninety-three genera were common in dental and ruminal fluid microbiomes and among them periodontal pathogens such as Fusobacterium, Prevotella, Porphyromonas and Actinomyces were recognized. Network analysis showed that dental microbiomes of clinically healthy animals tend to comprise a group of OTUs in homeostasis and when analyzed together, dental and ruminal fluid microbiomes of animals with periodontitis had almost twice the number of negative edges, indicating possible competition between bacteria and dysbiosis. The incisor dental and ruminal fluid microbiomes were dominated by a core community composed of members of the phyla Firmicutes and Bacteroidetes. Network results showed that members of the Prevotella genus stood out among the top five OTUs, with the largest number of hubs in the dental and ruminal microbiota of animals with periodontitis. Protein families linked to an inflammatory environment were predicted in the dental and ruminal microbiota of cattle with periodontitis. The dissimilarity between dental microbiomes, discriminating between healthy cattle and those with periodontitis and the identification of possible key pathogens, represent an important reference to elucidate the triggers involved in the etiopathogenesis of bovine periodontitis, and possibly in the development of measures to control the disease and reduce the pressures for deforestation.

• Item Type: Articles
• Additional Information: The authors acknowledge the USAID and the National Academies of Sciences, Engineering, and Medicine of the United States (NAS) for funding our research under PEER project 4–299, USAID agreement AID-OAA-A-11–00012.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Riggio, Professor Marcello
• Authors: Borsanelli, A. C., Athayde, F. R. F., Riggio, M. P., Brandt, B. W., Rocha, F. I., Jesus, E. C., Gaetti-Jardim, E., Schweitzer, C. M., and Dutra, I. S.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
• Journal Name: Frontiers in Microbiology
• Publisher: Frontiers Media
• ISSN: 1664-302X
• ISSN (Online): 1664-302X
• Copyright Holders: Copyright © 2022 Borsanelli, Athayde, Riggio, Brandt, Rocha, Jesus, Gaetti-Jardim, Schweitzer and Dutra
• First Published: First published in Frontiers in Microbiology 13: 936021
• Publisher Policy: Reproduced under a Creative Commons License