van Rossum, A.J., Brophy, P.M., Tait, A., Barrett, J. and Jefferies, J.R. (2001) Proteomic identification of glutathione S-transferases from the model nematode Caenorhabditis elegans. Proteomics, 1(11), pp. 1463-1468. (doi: 10.1002/1615-9861(200111)1)
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Abstract
Glutathione affinity chromatography and two-dimensional electrophoresis (2-DE) were used to purify glutathione binding proteins from Caenorhabditis elegans. All proteins identified after peptide mass fingerprinting using matrix-assisted laser desorption/ionization-time of flight were found to belong to the glutathione S-transferase (GST) superfamily. From the 26 individual spots identified, 12 different GSTs were isolated. Of these, five were found on the gel only once, whilst the remaining seven were represented by 21 separate spots. Most of the GSTs identified were of the nematode specific class, however, three Alpha class GSTs, a Pi and a Sigma class GST were also isolated.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Tait, Professor Andy |
| Authors: | van Rossum, A.J., Brophy, P.M., Tait, A., Barrett, J., and Jefferies, J.R. |
| College/School: | College of Medical Veterinary and Life Sciences |
| Journal Name: | Proteomics |
| ISSN: | 1615-9853 |
| ISSN (Online): | 1615-9861 |
| Published Online: | 21 November 2001 |
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