Planar junctionless field-effect transistor for detecting biomolecular interactions

Shukla, R. P., Bomer, J. G., Wijnperle, D., Kumar, N. , Georgiev, V. P. , Singh, A. C., Krishnamoorthy, S., García, C. P., Pud, S. and Olthuis, W. (2022) Planar junctionless field-effect transistor for detecting biomolecular interactions. Sensors, 22(15), 5783. (doi: 10.3390/s22155783)

Abstract

Label-free field-effect transistor-based immunosensors are promising candidates for proteomics and peptidomics-based diagnostics and therapeutics due to their high multiplexing capability, fast response time, and ability to increase the sensor sensitivity due to the short length of peptides. In this work, planar junctionless field-effect transistor sensors (FETs) were fabricated and characterized for pH sensing. The device with SiO2 gate oxide has shown voltage sensitivity of 41.8 ± 1.4, 39.9 ± 1.4, 39.0 ± 1.1, and 37.6 ± 1.0 mV/pH for constant drain currents of 5, 10, 20, and 50 nA, respectively, with a drain to source voltage of 0.05 V. The drift analysis shows a stability over time of −18 nA/h (pH 7.75), −3.5 nA/h (pH 6.84), −0.5 nA/h (pH 4.91), 0.5 nA/h (pH 3.43), corresponding to a pH drift of −0.45, −0.09, −0.01, and 0.01 per h. Theoretical modeling and simulation resulted in a mean value of the surface states of 3.8 × 1015/cm2 with a standard deviation of 3.6 × 1015/cm2. We have experimentally verified the number of surface sites due to APTES, peptide, and protein immobilization, which is in line with the theoretical calculations for FETs to be used for detecting peptide-protein interactions for future applications.

• Item Type: Articles
• Additional Information: Funding: This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 862539-Electromed-FET OPEN.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kumar, Dr Naveen and Georgiev, Professor Vihar
• Authors: Shukla, R. P., Bomer, J. G., Wijnperle, D., Kumar, N., Georgiev, V. P., Singh, A. C., Krishnamoorthy, S., García, C. P., Pud, S., and Olthuis, W.
• College/School: College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
• Journal Name: Sensors
• Publisher: MDPI
• ISSN: 1424-8220
• ISSN (Online): 1424-8220
• Published Online: 02 August 2022
• Copyright Holders: Copyright © 2022 by the authors
• First Published: First published in Sensors 22(15): 5783
• Publisher Policy: Reproduced under a Creative Commons licence