Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes

Battistella, E., Schniete, J., Wesencraft, K., Quintana, J. F. and McConnell, G. (2022) Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes. iScience, 25(9), 104797. (doi: 10.1016/j.isci.2022.104797) (PMID:36034214) (PMCID:PMC9404659)

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Abstract

Rapid imaging of large biological tissue specimens such as ultrathick sections of mouse brain cannot easily be performed with a standard microscope. Optical mesoscopy offers a solution, but thus far imaging has been too slow to be useful for routine use. We have developed two different illuminators for light-sheet mesoscopy with the Mesolens and we demonstrate their use in high-speed optical mesoscale imaging of large tissue specimens. The first light-sheet approach uses Gaussian optics and is straightforward to implement. It provides excellent lateral resolution and high-speed imaging, but the axial resolution is poor. The second light-sheet is a more complex Airy light-sheet that provides sub-cellular resolution in three dimensions that is comparable in quality to point-scanning confocal mesoscopy, but the light-sheet method of illuminating the specimen reduces the imaging time by a factor of 14. This creates new possibilities for high-content, higher-throughput optical bioimaging at the mesoscale.

Item Type:Articles
Additional Information:Eliana Battistella is supported by a Student Excellence Award from the University of Strathclyde. Jan Schniete is jointly funded by the University of Strathclyde, Glasgow, and the Hong Kong University of Science and Technology (HKUST), Hong Kong. Katrina Wesencraft is supported by the Medical Research Council and Engineering and Physical Sciences Research Council Center for Doctoral Training in Optical Medical Imaging, grant number EP/L016559/1, and by the Scottish Funding Council, grant number H14052/SIRL ID: 691. Juan F. Quintana is supported by a Sir Henry Wellcome postdoctoral fellowship (221640/Z/20/Z). Gail McConnell is supported by the Medical Research Council [MR/K015583/1] and Biotechnology & Biological Sciences Research Council [BB/P02565X/1, BB/T011602/1].
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Quintana, Dr Juan
Creator Roles:
Quintana, J. F.Validation, Resources, Writing – review and editing, Funding acquisition
Authors: Battistella, E., Schniete, J., Wesencraft, K., Quintana, J. F., and McConnell, G.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:iScience
Publisher:Elsevier (Cell Press)
ISSN:2589-0042
ISSN (Online):2589-0042
Published Online:21 July 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in iScience 25(9): 104797
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.15129/b44a4a73-ae23-46f2-b13f-5017955e8fe2

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
310358Molecular basis of pathogen-induced disruption of host circadian rhythmsJuan QuintanaWellcome Trust (WELLCOTR)221640/Z/20/ZMVLS - Polyomics Facility