Multiple insecticide resistance and first evidence of V410L kdr mutation in Aedes (Stegomyia) aegypti (Linnaeus) from Burkina Faso

Toé, H. K., Zongo, S., Guelbeogo, M. W., Kamgang, B., Viana, M. , Tapsoba, M., Sanou, A., Traoré, A., McCall, P. J. and Sagnon, N.'F. (2022) Multiple insecticide resistance and first evidence of V410L kdr mutation in Aedes (Stegomyia) aegypti (Linnaeus) from Burkina Faso. Medical and Veterinary Entomology, 36(3), pp. 309-319. (doi: 10.1111/mve.12602) (PMID:35869781)

Abstract

The response to recent dengue outbreaks in Burkina Faso was insecticide‐based, despite poor knowledge of the vector population's susceptibility to the insecticides used. Here, we report on the susceptibility to the main insecticide classes and identify important underlying mechanisms in Aedes aegypti populations in Ouagadougou and Banfora, in 2019 and 2020. Wild Ae. aegypti were tested as adults in WHO bioassays and then screened in real time melting curve qPCR analyses to genotype the F1534C, V1016I, and V410L Aedes kdr mutations. Ae. aegypti showed moderate resistance to 0.1% bendiocarb (80–95% survival post‐exposure), 0.8% Malathion (60–100%), 0.21% pirimiphos‐methyl (75% – 97%), and high resistance to 0.03% deltamethrin (20–70%). PBO pre‐exposure partially restored pyrethroid susceptibility. Genotyping detected high frequency of 1534C allele (0.92) and moderate 1016I (0.1–0.32). The V410L mutation was detected in Burkina Faso for the first time (frequency 0.1–0.36). Mosquitoes surviving 4 h exposure to 0.03% deltamethrin had significantly higher frequencies of the F1534C mutation than dead mosquitoes (0.70 vs. 0.96, p < 0.0001) and mosquitoes surviving 2 ‐ 4 h exposure had a significantly reduced life span. Ae. aegypti from Burkina Faso are resistant to multiple insecticide classes with multiple mechanisms involved, demonstrating the essential role of insecticide resistance monitoring within national dengue control programmes.

• Item Type: Articles
• Additional Information: This research leading to these results was (co-) funded by the Medical Research Council of the UK (grant number MR/P027873/1) through the Global Challenges Research Fund.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Viana, Dr Mafalda
• Authors: Toé, H. K., Zongo, S., Guelbeogo, M. W., Kamgang, B., Viana, M., Tapsoba, M., Sanou, A., Traoré, A., McCall, P. J., and Sagnon, N.'F.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Medical and Veterinary Entomology
• Publisher: Wiley
• ISSN: 0269-283X
• ISSN (Online): 1365-2915
• Published Online: 23 July 2022
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in Medical and Veterinary Entomology 36(3): 309-319
• Publisher Policy: Reproduced under a Creative Commons License