Material-driven fibronectin and vitronectin assembly enhances BMP-2 presentation and osteogenesis

Xiao, Y., Donnelly, H., Sprott, M. , Luo, J., Jayawarna, V., Lemgruber, L. , Tsimbouri, P. M. , Meek, R.M. D., Salmeron-Sanchez, M. and Dalby, M. J. (2022) Material-driven fibronectin and vitronectin assembly enhances BMP-2 presentation and osteogenesis. Materials Today Bio, 16, 100367. (doi: 10.1016/j.mtbio.2022.100367) (PMID:35937570) (PMCID:PMC9352550)

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Mesenchymal stem cell (MSC)-based tissue engineering strategies are of interest in the field of bone tissue regenerative medicine. MSCs are commonly investigated in combination with growth factors (GFs) and biomaterials to provide a regenerative environment for the cells. However, optimizing how biomaterials interact with MSCs and efficiently delivering GFs, remains a challenge. Here, via plasma polymerization, tissue culture plates are coated with a layer of poly (ethyl acrylate) (PEA), which is able to spontaneously permit fibronectin (FN) to form fibrillar nanonetworks. However, vitronectin (VN), another important extracellular matrix (ECM) protein forms multimeric globules on the polymer, thus not displaying function groups to cells. Interestingly, when FN and VN are co-absorbed onto PEA surfaces, VN can be entrapped within the FN fibrillar nanonetwork in the monomeric form providing a heterogeneous, open ECM network. The combination of FN and VN enhances MSC adhesion and leads to enhanced GF binding; here we demonstrate this with bone morphogenetic protein-2 (BMP2). Moreover, MSC differentiation into osteoblasts is enhanced, with elevated expression of osteopontin (OPN) and osteocalcin (OCN) quantified by immunostaining, and increased mineralization observed by von Kossa staining. Osteogenic intracellular signalling is also induced, with increased activity in the SMAD pathway. The study emphasizes the need of recapitulating the complexity of native ECM to achieve optimal cell-material interactions.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Dalby, Professor Matthew and Tsimbouri, Dr Monica and Xiao, Yinbo and Lemgruber Soares, Dr Leandro and Luo, Mr Jiajun and Jayawarna, Dr Vineetha and Meek, Professor Dominic and Sprott, Dr Mark and Donnelly, Dr Hannah
Authors: Xiao, Y., Donnelly, H., Sprott, M., Luo, J., Jayawarna, V., Lemgruber, L., Tsimbouri, P. M., Meek, R.M. D., Salmeron-Sanchez, M., and Dalby, M. J.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Materials Today Bio
ISSN (Online):2590-0064
Published Online:19 July 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Materials Today Bio 16: 100367
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173192Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - Biomedical Engineering