Characterisation of North American Brucella isolates from marine mammals

Whatmore, A. M. et al. (2017) Characterisation of North American Brucella isolates from marine mammals. PLoS ONE, 12(9), e0184758. (doi: 10.1371/journal.pone.0184758) (PMID:28934239) (PMCID:PMC5608248)

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Extension of known ecological niches of Brucella has included the description of two novel species from marine mammals. Brucella pinnipedialis is associated predominantly with seals, while two major Brucella ceti clades, most commonly associated with porpoises or dolphins respectively, have been identified. To date there has been limited characterisation of Brucella isolates obtained from marine mammals outside Northern European waters, including North American waters. To address this gap, and extend knowledge of the global population structure and host associations of these Brucella species, 61 isolates from marine mammals inhabiting North American waters were subject to molecular and phenotypic characterisation enabling comparison with existing European isolates. The majority of isolates represent genotypes previously described in Europe although novel genotypes were identified in both B. ceti clades. Harp seals were found to carry B. pinnipedialis genotypes previously confined to hooded seals among a diverse repertoire of sequence types (STs) associated with this species. For the first time Brucella isolates were characterised from beluga whales and found to represent a number of distinct B. pinnipedialis genotypes. In addition the known host range of ST27 was extended with the identification of this ST from California sea lion samples. Finally the performance of the frequently used diagnostic tool Bruce-ladder, in differentiating B. ceti and B. pinnipedialis, was critically assessed based on improved knowledge of the global population structure of Brucella associated with marine mammals.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Davison, Mr Nick
Creator Roles:
Davison, N. J.Resources, Writing – review and editing
Authors: Whatmore, A. M., Dawson, C., Muchowski, J., Perrett, L. L., Stubberfield, E., Koylass, M., Foster, G., Davison, N. J., Quance, C., Sidor, I. F., Field, C. L., and St. Leger, J.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN (Online):1932-6203
Copyright Holders:This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose
First Published:First published in PLoS ONE 12(9): e0184758
Publisher Policy:Reproduced under a Creative Commons License

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