Visualizing synaptic connectivity using confocal and electron microscopy: neuroanatomical approaches to define spinal circuits

Hughes, D. I. and Todd, A. J. (2022) Visualizing synaptic connectivity using confocal and electron microscopy: neuroanatomical approaches to define spinal circuits. In: Contemporary Approaches to the Study of Pain. Series: Neuromethods, 178. Humana: New York, pp. 181-201. ISBN 9781071620380 (doi: 10.1007/978-1-0716-2039-7_11)

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Abstract

Visualizing the synaptic connectivity of identified neuronal populations is an essential part of defining neuronal circuits in the central nervous system. In our laboratory, we have optimized several histological approaches that are compatible with confocal and transmission electron microscopy (TEM) to study the anatomical relationships between sensory afferents, interneurons, and projection neurons in the spinal dorsal horn. The aims of this chapter are to provide detailed descriptions of some of these protocols, to highlight both advantages and potential difficulties that may be encountered when using these approaches, and to reference several studies where these have been applied to study spinal circuits in normal and chronic pain states.

Item Type:Book Sections
Status:Published
Glasgow Author(s) Enlighten ID:Hughes, Dr David I and Todd, Professor Andrew
Authors: Hughes, D. I., and Todd, A. J.
College/School:College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
Publisher:Humana
ISSN:0893-2336
ISBN:9781071620380
Published Online:27 May 2022

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