Koester, A. M. et al. (2022) EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes. Bioscience Reports, 42(7), BSR20221181. (doi: 10.1042/BSR20221181) (PMID:35735144) (PMCID:PMC9272592)
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Abstract
Insulin stimulates glucose transport in muscle and adipocytes. This is achieved by regulated delivery of intracellular glucose transporter (GLUT4)-containing vesicles to the plasma membrane where they dock and fuse, resulting in increased cell surface GLUT4 levels. Recent work identified a potential further regulatory step, in which insulin increases the dispersal of GLUT4 in the plasma membrane away from the sites of vesicle fusion. EFR3 is a scaffold protein that facilitates localisation of phosphatidylinositol 4-kinase type IIIa to the cell surface. Here we show that knockdown of EFR3 or phosphatidylinositol 4-kinase type IIIa impairs insulin-stimulated glucose transport in adipocytes. Using direct stochastic reconstruction microscopy, we also show that EFR3 knockdown impairs insulin stimulated GLUT4 dispersal in the plasma membrane. We propose that EFR3 plays a previously unidentified role in controlling insulin-stimulated glucose transport by facilitating dispersal of GLUT4 within the plasma membrane.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Koester, Anna and Gould, Professor Gwyn and Black, Hannah Lucy and Cutiongco, Ms Marie and LAIDLAW, Kamilla and Bryant, Dr Nia and Gadegaard, Professor Nikolaj and Stirrat, Mrs Laura and Morris, Silke |
| Authors: | Koester, A. M., Geiser, A., Laidlaw, K. M.E., Morris, S., Cutiongco, M. F.A., Stirrat, L., Gadegaard, N., Boles, E., Black, H. L., Bryant, N., and Gould, G. W. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Bioscience Reports |
| Publisher: | Portland Press |
| ISSN: | 0144-8463 |
| ISSN (Online): | 1573-4935 |
| Published Online: | 23 June 2022 |
| Copyright Holders: | Copyright © 2022 The Authors |
| First Published: | First published in Bioscience Reports 42(7): BSR20221181 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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