Tracking molecular diffusion across biomaterials' interfaces using stimulated Raman scattering

Cui, H. , Glidle, A. and Cooper, J. M. (2022) Tracking molecular diffusion across biomaterials' interfaces using stimulated Raman scattering. ACS Applied Materials and Interfaces, 14(28), pp. 31586-31593. (doi: 10.1021/acsami.2c04444) (PMID:35801584)

Abstract

The determination of molecular diffusion across biomaterial interfaces, including those involving hydrogels and tissues remains important, underpinning the understanding of a broad range of processes including, for example, drug delivery. Current techniques using Raman spectroscopy have previously been established as a method to quantify diffusion coefficients, although when using spontaneous Raman spectroscopy, the signal can be weak and dominated by interferences such as background fluorescence (including biological autofluoresence). To overcome these issues, we demonstrate the use of the stimulated Raman scattering technique to obtain measurements in soft tissue samples that have good signal-to-noise ratios and are largely free from fluorescence interference. As a model illustration of a small metabolite/drug molecule being transported through tissue, we use deuterated (d7-) glucose and monitor the Raman C–D band in a spectroscopic region free from other Raman bands. The results show that although mass transport follows a diffusion process characterized by Fick’s laws within hydrogel matrices, more complex mechanisms appear within tissues.

• Item Type: Articles
• Additional Information: The authors gratefully acknowledge the support of the Engineering and Physical Sciences Research Council (EP/P001114/1) and National Nature Science Foundation of China (NSFC)(No. 62105021).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cooper, Professor Jonathan and Cui, Dr Han and Glidle, Dr Andrew
• Authors: Cui, H., Glidle, A., and Cooper, J. M.
• College/School: College of Science and Engineering > School of Engineering; College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: ACS Applied Materials and Interfaces
• Publisher: American Chemical Society
• ISSN: 1944-8244
• ISSN (Online): 1944-8252
• Published Online: 08 July 2022
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in ACS Applied Materials and Interfaces 14(28): 31586-31593
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
173192		Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicine	Manuel Salmeron-Sanchez	Engineering and Physical Sciences Research Council (EPSRC)	EP/P001114/1	ENG - Biomedical Engineering