Tracking molecular diffusion across biomaterials' interfaces using stimulated Raman scattering

Cui, H. , Glidle, A. and Cooper, J. M. (2022) Tracking molecular diffusion across biomaterials' interfaces using stimulated Raman scattering. ACS Applied Materials and Interfaces, 14(28), pp. 31586-31593. (doi: 10.1021/acsami.2c04444) (PMID:35801584)

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The determination of molecular diffusion across biomaterial interfaces, including those involving hydrogels and tissues remains important, underpinning the understanding of a broad range of processes including, for example, drug delivery. Current techniques using Raman spectroscopy have previously been established as a method to quantify diffusion coefficients, although when using spontaneous Raman spectroscopy, the signal can be weak and dominated by interferences such as background fluorescence (including biological autofluoresence). To overcome these issues, we demonstrate the use of the stimulated Raman scattering technique to obtain measurements in soft tissue samples that have good signal-to-noise ratios and are largely free from fluorescence interference. As a model illustration of a small metabolite/drug molecule being transported through tissue, we use deuterated (d7-) glucose and monitor the Raman C–D band in a spectroscopic region free from other Raman bands. The results show that although mass transport follows a diffusion process characterized by Fick’s laws within hydrogel matrices, more complex mechanisms appear within tissues.

Item Type:Articles
Additional Information:The authors gratefully acknowledge the support of the Engineering and Physical Sciences Research Council (EP/P001114/1) and National Nature Science Foundation of China (NSFC)(No. 62105021).
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Cui, Dr Han and Glidle, Dr Andrew
Authors: Cui, H., Glidle, A., and Cooper, J. M.
College/School:College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:ACS Applied Materials and Interfaces
Publisher:American Chemical Society
ISSN (Online):1944-8252
Published Online:08 July 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in ACS Applied Materials and Interfaces 14(28): 31586-31593
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173192Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - Biomedical Engineering