The neutralizing antibody responses of individuals that spontaneously resolve hepatitis C virus infection

Cowton, V. M., Dunlop, J. I., Cole, S. J., Swann, R. E. and Patel, A. H. (2022) The neutralizing antibody responses of individuals that spontaneously resolve hepatitis C virus infection. Viruses, 14(7), 1391. (doi: 10.3390/v14071391) (PMID:35891372) (PMCID:PMC9318067)

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Hepatitis C virus (HCV) infection is a major global health problem. In the majority of cases the virus is not cleared by the host immune response and progresses to chronic infection. Studies of the neutralizing antibody responses in individuals that naturally clear infection are limited. Understanding what constitutes a successful antibody response versus one that has ‘failed’ and resulted in chronic infection is important to understand what type of antibody response would need to be elicited by a protective vaccine. Samples from spontaneous clearers are difficult to obtain therefore studies are often limited. In our study through HCV Research UK, we had access to a cohort of over 200 samples. We identified the samples that contained HCV neutralizing antibodies using ELISA and HCV pseudoparticle (HCVpp) assays. We then utilised mutagenesis and cross-competition analysis to determine the profile of the neutralizing antibody responses. In addition, we analysed a cohort of samples from chronic infection using the same techniques to enable direct comparison of the antibody profiles observed in both cohorts. We conclude that similar profiles are present in both cohorts indicating that it is not the neutralizing antibody response per se that determines the outcome of infection. These data will provide useful information for future HCV vaccine design.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Swann, Dr Rachael and Patel, Professor Arvind and Dunlop, Dr James and Cowton, Dr Vanessa and Cole, Mrs Sarah
Creator Roles:
Cowton, V. M.Conceptualization, Methodology, Investigation, Writing – original draft, Writing – review and editing, Supervision
Dunlop, J. I.Conceptualization, Methodology, Investigation, Writing – review and editing, Supervision
Cole, S. J.Investigation, Writing – review and editing
Swann, R. E.Investigation, Resources, Writing – review and editing
Patel, A. H.Conceptualization, Writing – review and editing, Project administration, Supervision, Funding acquisition
Authors: Cowton, V. M., Dunlop, J. I., Cole, S. J., Swann, R. E., and Patel, A. H.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Viruses
ISSN (Online):1999-4915
Published Online:25 June 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Viruses 14(7): 1391
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172630Basis of the host range and tissue tropism for hepatitis C virusArvind PatelMedical Research Council (MRC)MC_UU_12014/2III - Centre for Virus Research
190654Establishment of a Resource for Long-Term Study of Hepatitis C Virus Infection in the UKJohn McLauchlanMedical Research Foundation (MEDRESFO)C0365III-MRC-GU Centre for Virus Research