Cowton, V. M., Dunlop, J. I., Cole, S. J., Swann, R. E. and Patel, A. H. (2022) The neutralizing antibody responses of individuals that spontaneously resolve hepatitis C virus infection. Viruses, 14(7), 1391. (doi: 10.3390/v14071391) (PMID:35891372) (PMCID:PMC9318067)
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Abstract
Hepatitis C virus (HCV) infection is a major global health problem. In the majority of cases the virus is not cleared by the host immune response and progresses to chronic infection. Studies of the neutralizing antibody responses in individuals that naturally clear infection are limited. Understanding what constitutes a successful antibody response versus one that has ‘failed’ and resulted in chronic infection is important to understand what type of antibody response would need to be elicited by a protective vaccine. Samples from spontaneous clearers are difficult to obtain therefore studies are often limited. In our study through HCV Research UK, we had access to a cohort of over 200 samples. We identified the samples that contained HCV neutralizing antibodies using ELISA and HCV pseudoparticle (HCVpp) assays. We then utilised mutagenesis and cross-competition analysis to determine the profile of the neutralizing antibody responses. In addition, we analysed a cohort of samples from chronic infection using the same techniques to enable direct comparison of the antibody profiles observed in both cohorts. We conclude that similar profiles are present in both cohorts indicating that it is not the neutralizing antibody response per se that determines the outcome of infection. These data will provide useful information for future HCV vaccine design.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Swann, Dr Rachael and Patel, Professor Arvind and Dunlop, Dr James and Cowton, Dr Vanessa and Cole, Ms Sarah |
| Creator Roles: | Cowton, V. M.Conceptualization, Methodology, Investigation, Writing – original draft, Writing – review and editing, Supervision Dunlop, J. I.Conceptualization, Methodology, Investigation, Writing – review and editing, Supervision Cole, S. J.Investigation, Writing – review and editing Swann, R. E.Investigation, Resources, Writing – review and editing Patel, A. H.Conceptualization, Writing – review and editing, Project administration, Supervision, Funding acquisition |
| Authors: | Cowton, V. M., Dunlop, J. I., Cole, S. J., Swann, R. E., and Patel, A. H. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Journal Name: | Viruses |
| Publisher: | MDPI |
| ISSN: | 1999-4915 |
| ISSN (Online): | 1999-4915 |
| Published Online: | 25 June 2022 |
| Copyright Holders: | Copyright © 2022 The Authors |
| First Published: | First published in Viruses 14(7): 1391 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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Funder and Project Information
Funder and Project Information