TANDEM ZINC-FINGER/PLUS3 regulates phytochrome B abundance and signaling to fine-tune hypocotyl growth

Fang, W., Vellutini, E., Perrella, G. and Kaiserli, E. (2022) TANDEM ZINC-FINGER/PLUS3 regulates phytochrome B abundance and signaling to fine-tune hypocotyl growth. Plant Cell, 34(11), pp. 4213-4231. (doi: 10.1093/plcell/koac236) (PMID:35929801) (PMCID:PMC9614508)

Abstract

TANDEM ZINC-FINGER/PLUS3 (TZP) is a transcriptional regulator that acts at the crossroads of light and photoperiodic signaling. Here, we unveil a role for TZP in fine-tuning hypocotyl elongation under red light and long-day conditions. We provide genetic evidence for a synergistic action between TZP and PHOTOPERIODIC CONTROL OF HYPOCOTYL 1 (PCH1) in regulating the protein abundance of PHYTOCHROME INTERACTING FACTOR 4 (PIF4) and downstream gene expression in response to red light and long days (LDs). Furthermore, we show that TZP is a positive regulator of the red/far-red light receptor and thermosensor phytochrome B (phyB) by promoting phyB protein abundance, nuclear body formation, and signaling. Our data therefore assign a function to TZP in regulating two key red light signaling components, phyB and PIF4, but also uncover a new role for PCH1 in regulating hypocotyl elongation in LDs. Our findings provide a framework for the understanding of the mechanisms associated with the TZP signal integration network and their importance for optimizing plant growth and adaptation to a changing environment.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Fang, Weiwei and Perrella, Dr Giorgio and Kaiserli, Dr Eirini and Vellutini, Miss Elisa
• Authors: Fang, W., Vellutini, E., Perrella, G., and Kaiserli, E.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Plant Cell
• Publisher: Oxford University Press
• ISSN: 1040-4651
• ISSN (Online): 1532-298X
• Published Online: 05 August 2022
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in Plant Cell 34(11): 4213-4231
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
171989		Transcriptional regulation of plant growth in nuclear microdomains	Eirini Kaiserli	Biotechnology and Biological Sciences Research Council (BBSRC)	BB/M023079/1	Institute of Molecular, Cell & Systems Biology