Williams, J. A., Huesa, C., Windmill, J. F. C., Purcell, M., Reid, S., Coupaud, S. and Riddell, J. S. (2022) Spatiotemporal responses of trabecular and cortical bone to complete spinal cord injury in skeletally mature rats. Bone Reports, 16, 101592. (doi: 10.1016/j.bonr.2022.101592) (PMID:35637974) (PMCID:PMC9142855)
![[img]](https://eprints.gla.ac.uk/style/images/fileicons/text.png) |
Text
273207.pdf - Published Version Available under License Creative Commons Attribution. 6MB |
Abstract
Objective: Characterise the spatiotemporal responses of trabecular and cortical bone to complete spinal cord injury (SCI) in the skeletally mature rat in the acute (4-week) period following injury. Methods: The spinal cord of 5-month old male rats was transected at the T9 level. Outcome measures were assessed using micro-computed tomography, three-point bending and serum markers at 1-, 2-, and 4-weeks post-transection. Comparison was made with time-0 and sham animals. Results: Lower levels of circulating serum bone formation markers and higher bone resorption markers suggested uncoupled bone turnover as early at 1-week post-transection. Micro-computed tomography showed metaphyseal and epiphyseal trabecular bone loss was observed only at 4-weeks post-transection. The bone loss was site-specific with a more severe reduction in trabecular BV/TV observed in the metaphyseal (50%) relative to epiphyseal (19%) region. Metaphyseal trabecular bone exhibited a 54% reduction in connectivity density while the epiphyseal trabecular bone was unaffected. Cortical bone deficits were not seen over the time periods examined. Conclusions: The study demonstrates that the skeletally mature spinal cord transected rat model replicates the biphasic pattern of osteoporotic changes observed in the human SCI population, providing a relevant model for testing the efficacy of interventions against SCI-induced osteoporosis.
| Item Type: | Articles |
|---|---|
| Additional Information: | The study was supported by funding provided by the Science and Technology Facilities Council (STFC) Challenge Led Applied Systems Programme (ST/S000968/1) and (ST/S000852/1) and in part by the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement no. 615030. Carmen Huesa was supported by the Versus Arthritis early career fellowship (grant no. 22483). |
| Keywords: | Osteoporosis, Mechanical testing, Skeletally mature, Spinal cord injury, Bone morphometry, microCT |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Huesa, Dr Carmen and Riddell, Professor John |
| Authors: | Williams, J. A., Huesa, C., Windmill, J. F. C., Purcell, M., Reid, S., Coupaud, S., and Riddell, J. S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience |
| Research Centre: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology |
| Journal Name: | Bone Reports |
| Publisher: | Elsevier |
| ISSN: | 2352-1872 |
| ISSN (Online): | 2352-1872 |
| Published Online: | 21 May 2022 |
| Copyright Holders: | Copyright © 2022 The Authors |
| First Published: | First published in Bone Reports 16: 101592 |
| Publisher Policy: | Reproduced under a Creative Commons licence |
University Staff: Request a correction | Enlighten Editors: Update this record
Funder and Project Information
Funder and Project Information