Walker, L. et al. (2022) Characterization of an alloherpesvirus from wild lake sturgeon Acipenser fulvescens in Wisconsin (USA). Diseases of Aquatic Organisms, 149, pp. 83-96. (doi: 10.3354/dao03661) (PMID:35686452)
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Abstract
In the spring of 2017, 2 adult lake sturgeon (LS) Acipenser fulvescens captured from the Wolf River, Wisconsin (USA), presented with multiple cutaneous plaques that, upon microscopic examination, indicated proliferative epidermitis. Ultrastructural examination of affected keratinocytes revealed particles in the nucleus having a morphology typical of herpesviruses. A degenerate PCR assay targeting the DNA polymerase catalytic subunit (pol) gene of large double-stranded DNA viruses generated amplicons of the anticipated size from skin samples, and sequences of amplicons confirmed the presence of a novel alloherpesvirus (lake sturgeon herpesvirus, LSHV) related to acipenserid herpesvirus 1 (AciHV1). The complete genome (202660 bp) of this virus was sequenced using a MiSeq System, and phylogenetic analyses substantiated the close relationship to AciHV1. A PCR assay targeting the LSHV DNA packaging terminase subunit 1 (ter1) gene demonstrated the presence of the virus in 39/42 skin lesion samples collected from wild LS captured in 2017-2019 and 2021 in 4/4 rivers in Wisconsin. Future efforts to isolate LSHV in cell culture would facilitate challenge studies to determine the disease potential of the virus.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Davison, Professor Andrew and Kerr, Ms Karen |
| Authors: | Walker, L., Subramaniam, K., Viadanna, P. H.O., Vann, J. A., Marcquenski, S., Godard, D., Kieran, E., Frasca Jr., S., Popov, V. L., Kerr, K., Davison, A. J., and Waltzek, T. B. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Diseases of Aquatic Organisms |
| Publisher: | Inter Research |
| ISSN: | 0177-5103 |
| ISSN (Online): | 1616-1580 |
| Published Online: | 02 June 2022 |
| Copyright Holders: | Copyright © 2022 The Authors |
| First Published: | First published in Diseases of Aquatic Organisms 149: 83-96 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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