Cell viability assays for Candida auris

Brown, J. L. , Short, B., Ware, A., Sherry, L. , Kean, R. and Ramage, G. (2022) Cell viability assays for Candida auris. In: Lorenz, A. (ed.) Candida auris. Series: Methods in molecular biology (2517). Humana: New York, pp. 129-153. ISBN 9781071624166 (doi: 10.1007/978-1-0716-2417-3_10)

Abstract

Cell viability assays are useful for assessing the efficacy of antifungal therapeutics and disinfection strategies in vitro. In recent years these assays have been fundamental for the testing of conventional and novel therapies against the nosocomial fungal pathogen Candida auris. Here we provide detailed descriptions of methods for assessing cellular viability of Candida auris in vitro, such as metabolic assays (XTT and resazurin), colony-forming unit counting, live/dead quantitative PCR, and fluorescent staining for microscopic analyses.

• Item Type: Book Sections
• Additional Information: Online ISBN: 9781071624173.
• Status: Published
• Glasgow Author(s) Enlighten ID: Short, Mr Bryn and Kean, Mr Ryan and Sherry, Dr Leighann and Brown, Dr Jason and Ramage, Professor Gordon
• Authors: Brown, J. L., Short, B., Ware, A., Sherry, L., Kean, R., and Ramage, G.
• College/School: College of Medical Veterinary and Life Sciences > School of Life Sciences; College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
• Publisher: Humana
• ISSN: 1064-3745
• ISBN: 9781071624166
• Copyright Holders: Copyright © 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
• First Published: First published in Candida auris 129-153
• Publisher Policy: Reproduced in accordance with the publisher copyright policy