Medina-Ruiz, L. et al. (2022) Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice. eLife, 11, e72418. (doi: 10.7554/eLife.72418) (PMID:35699420) (PMCID:PMC9236609)
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Abstract
Inflammatory chemokines and their receptors are central to the development of inflammatory/immune pathologies. The apparent complexity of this system, coupled with lack of appropriate in vivo models, has limited our understanding of how chemokines orchestrate inflammatory responses and has hampered attempts at targeting this system in inflammatory disease. Novel approaches are therefore needed to provide crucial biological, and therapeutic, insights into the chemokine-chemokine receptor family. Here, we report the generation of transgenic multi-chemokine receptor reporter mice in which spectrally distinct fluorescent reporters mark expression of CCRs 1, 2, 3, and 5, key receptors for myeloid cell recruitment in inflammation. Analysis of these animals has allowed us to define, for the first time, individual and combinatorial receptor expression patterns on myeloid cells in resting and inflamed conditions. Our results demonstrate that chemokine receptor expression is highly specific, and more selective than previously anticipated.
| Item Type: | Articles |
|---|---|
| Additional Information: | Funding: Wellcome Trust (217093/Z/19/Z); Medical Research Council (MRV0109721); Max-Planck-Institute for Cell Biology and Genetics (open access funding); University of Glasgow. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Love, Samantha and Wilson, Dr Gillian and Hughes, Dr Catherine and Vidler, Ms Francesca and Dyer, Dr Douglas and Schuette, Dr Fabian and Bartolini, Mr Robin and Medina-Ruiz, Dr Laura and Graham, Professor Gerard |
| Creator Roles: | Medina-Ruiz, L.Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Supervision, Writing – original draft, Writing – review and editing Bartolini, R.Conceptualization, Data curation, Formal analysis, Investigation, Supervision, Writing – original draft Wilson, G.Formal analysis, Investigation, Methodology Dyer, D.Conceptualization, Data curation, Formal analysis, Investigation, Supervision, Writing – original draft Vidler, F.Data curation, Formal analysis, Investigation, Writing – original draft Hughes, C.Data curation, Formal analysis, Project administration, Writing – original draft Schuette, F.Conceptualization, Formal analysis, Investigation, Writing – original draft Love, S.Data curation, Formal analysis, Investigation, Writing – original draft Graham, G.Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Project administration, Supervision, Writing – original draft, Writing – review and editing |
| Authors: | Medina-Ruiz, L., Bartolini, R., Wilson, G. J., Dyer, D. P., Vidler, F., Hughes, C. E., Schuette, F., Love, S., Fu, J., Stewart, A. F., and Graham, G. J. |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Research Centre: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology |
| Journal Name: | eLife |
| Publisher: | eLife Sciences Publications |
| ISSN: | 2050-084X |
| ISSN (Online): | 2050-084X |
| Copyright Holders: | Copyright © 2022 The Authors |
| First Published: | First published in eLife 11:e72418 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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