Whatmore, A. M. et al. (2014) Brucella papionis sp. nov., isolated from baboons (Papio spp.). International Journal of Systematic and Evolutionary Microbiology, 64(12), pp. 4120-4128. (doi: 10.1099/ijs.0.065482-0) (PMID:25242540) (PMCID:PMC4811636)
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Abstract
Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60T and F8/08-61) isolated from clinical specimens obtained from baboons (Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, were assigned to the genus Brucella . This placement was confirmed by extended multilocus sequence analysis (MLSA), where both strains possessed identical sequences, and whole-genome sequencing of a representative isolate. All of the above analyses suggested that the two strains represent a novel lineage within the genus Brucella . The strains also possessed a unique profile when subjected to the phenotyping approach classically used to separate species of the genus Brucella, reacting only with Brucella A monospecific antiserum, being sensitive to the dyes thionin and fuchsin, being lysed by bacteriophage Wb, Bk2 and Fi phage at routine test dilution (RTD) but only partially sensitive to bacteriophage Tb, and with no requirement for CO2 and no production of H2S but strong urease activity. Biochemical profiling revealed a pattern of enzyme activity and metabolic capabilities distinct from existing species of the genus Brucella . Molecular analysis of the omp2 locus genes showed that both strains had a novel combination of two highly similar omp2b gene copies. The two strains shared a unique fingerprint profile of the multiple-copy Brucella-specific element IS711. Like MLSA, a multilocus variable number of tandem repeat analysis (MLVA) showed that the isolates clustered together very closely, but represent a distinct group within the genus Brucella . Isolates F8/08-60T and F8/08-61 could be distinguished clearly from all known species of the genus Brucella and their biovars by both phenotypic and molecular properties. Therefore, by applying the species concept for the genus Brucella suggested by the ICSP Subcommittee on the Taxonomy of Brucella, they represent a novel species within the genus Brucella , for which the name Brucella papionis sp. nov. is proposed, with the type strain F8/08-60T ( = NCTC 13660T = CIRMBP 0958T).
| Item Type: | Articles |
|---|---|
| Additional Information: | This investigation used resources that were supported by the Southwest National Primate Research Center grant P51 RR013986 from the National Center for Research Resources, National Institutes of Health, and which are currently supported by the Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health through P51 OD011133 and conducted in facilities constructed with support from ORIP through grants 1 C06 RR014578 and 1 C06 RR015456. The work of S. Al D. was supported by a grant from the Federal Institute for Risk Assessment (BfR project no. 1322-503). The work of A. C., M. Z. and G. V. benefited from a grant from the French ‘Direction Ge´ne´rale de l’Armement’ (grant Brucella REI2010 34 0003), which supports the development of tools for the tracing of dangerous pathogens. Brucellosis research and surveillance activities at AHVLA are funded by the UK Department of Environment, Food and Rural Affairs (Defra). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Davison, Mr Nick |
| Authors: | Whatmore, A. M., Davison, N., Cloeckaert, A., Dahouk, S. A., Zygmunt, M. S., Brew, S. D., Perrett, L. L., Koylass, M. S., Vergnaud, G., Quance, C., Scholz, H. C., Dick Jr., E. J., Hubbard, G., and Schlabritz-Loutsevitch, N. E. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | International Journal of Systematic and Evolutionary Microbiology |
| Publisher: | Microbiology Society |
| ISSN: | 1466-5026 |
| ISSN (Online): | 1466-5034 |
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