Ma, L. et al. (2022) Effect of Astragalus membranaceus root on the serum metabolome of preweaning dairy calves. Agriculture, 12(6), 744. (doi: 10.3390/agriculture12060744)
![[img]](https://eprints.gla.ac.uk/style/images/fileicons/text.png) |
Text
271760.pdf - Published Version Available under License Creative Commons Attribution. 2MB |
Abstract
The effect of the Astragalus membranaceus root (AMR) on the serum metabolic profiles of preweaning calf were investigated in this study. Sixteen preweaning Holstein calves were randomly allocated into two groups with eight calves per group, and offered a control basal diet (CON group) or supplemented with 20 g superfine powder of AMR (AMR group) for 14 d. Serum samples were collected from calves on day 0, 7 and 14. Serum albumin, globulin, total protein, glucose, reduced glutathione and superoxide dismutase were evaluated. Serum metabolic profiling was analyzed using ultra-high-performance liquid chromatography−time-of-flight mass spectrometry. The results demonstrated that there were no significantly difference of total protein, glucose, reduced glutathione and superoxide dismutase between the CON group and AMR group (p > 0.05), while within the CON group and AMR group, serum glucose showed a continuous upward trend from 0 d to 14 d (p 0.001). Untargeted metabolomics analysis found the metabolism of preweaning calf was considerably changed during growth, mainly including amino acid metabolism and carbohydrate metabolism and showed an increasing in protein synthesis and gluconeogenesis. 19 differential metabolites have been screened after supplementing AMR for 14 d, nine of which were up-regulated, including ornithine, L-pyroglutamic acid, L-proline and D-proline, and 10 down-regulated, containing L-kynurenine, 5-hydroxyindoleacetate, linoleic acid and 4-pyridoxic acid. Pathway analysis found these metabolites mainly participated in three primary pathways: arginine and proline metabolism, tryptophan metabolism and glutathione metabolism (p 0.05), while linoleic acid metabolism and vitamin B6 metabolism were also enriched (0.1 p 0.05). Such metabolic changes reflected the enhancement of the antioxidant and anti-inflammatory capacity of preweaning calves.
| Item Type: | Articles |
|---|---|
| Additional Information: | This study was funded by the Key R&D Program Supported by the Department of Science and Technology of Gansu Province (No. 20YF8NA029) and the Sichuan Department of Science and Technology Support Project (2019YJ0671). |
| Keywords: | Astragalus membranaceus root (AMR), preweaning dairy calves, metabolomics, antioxidant and anti-inflammatory ability. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Huang, Yixin |
| Creator Roles: | |
| Authors: | Ma, L., Luo, Z., Chen, J., Du, Z., Zhou, T., Huang, Y., Yao, X., Shen, L., Yu, S., Shi, X., and Cao, S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Agriculture |
| Publisher: | MDPI |
| ISSN: | 2077-0472 |
| ISSN (Online): | 2077-0472 |
| Published Online: | 24 May 2022 |
| Copyright Holders: | Copyright © 2022 The Authors |
| First Published: | First published in Agriculture 12(6): 744 |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details