Living biointerfaces for the maintenance of mesenchymal stem cell phenotypes

Petaroudi, M., Rodrigo-Navarro, A. , Dobre, O. , Dalby, M. J. and Salmeron-Sanchez, M. (2022) Living biointerfaces for the maintenance of mesenchymal stem cell phenotypes. Advanced Functional Materials, 32(32), 2203352. (doi: 10.1002/adfm.202203352)

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Living interfaces are established as a novel class of active materials that aim to provide an alternative to traditional static cell culture methods by enabling users to accurately control cell behaviour in a precise, dynamic, and reliable system-internal manner. To this day, the only reported biointerface has been a coculture between a biofilm of nonpathogenic genetically engineered bacteria and mammalian cells, where the recombinant proteins produced by the bacteria directly influence cell behaviour. In this work, a biointerface is presented between Lactococcus lactis (L. lactis) and human mesenchymal stem cells (hMSCs). L. lactis have been engineered to produce human C-X-C motif chemokine ligand 12, thrombopoietin, vascular cell adhesion protein 1, and the 7th–10th type III domains of human fibronectin, with the aim of recreating the native bone marrow conditions ex vivo. This active microenvironment has been shown to maintain key hMSC stemness markers, preventing their osteogenic and adipogenic differentiation, and maintaining high stem cell viability and physiological cell-to-substrate adhesion dynamics. This work presents proof of concept data that hMSC stemness can be regulated by living materials, using a system based on the symbiotic interaction between different engineered bacteria and mammalian cells.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Rodrigo-Navarro, Mr Aleixandre and Dobre, Dr Oana and Petaroudi, Dr Michaela and Dalby, Professor Matthew
Authors: Petaroudi, M., Rodrigo-Navarro, A., Dobre, O., Dalby, M. J., and Salmeron-Sanchez, M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Advanced Functional Materials
ISSN (Online):1616-3028
Published Online:31 May 2022
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Advanced Functional Materials 32(32): 2203352
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173192Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - Biomedical Engineering