Panagiotou, N. , McGuinness, D., Jaminon, A. M.G., Mees, B., Selman, C. , Schurgers, L. and Shiels, P. G. (2023) Microvesicle-mediated tissue regeneration mitigates the effects of cellular ageing. Cells, 12(13), 1707. (doi: 10.3390/cells12131707) (PMID:37443741) (PMCID:PMC10340655)
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Abstract
Extracellular vesicles (EVs), comprising microvesicles (MVs) and exosomes (Exos), are membranous vesicles secreted by cells which mediate the repair of cellular and tissue damage via paracrine mechanisms. The action of EVs under normative and morbid conditions in the context of ageing remains largely unexplored. We demonstrate that MVs, but not Exos, from Pathfinder cells (PCs), a putative stem cell regulatory cell type, enhance the repair of human dermal fibroblast (HDF) and mesenchymal stem cell (MSC) co-cultures, following both mechanical and genotoxic stress. Critically, this effect was found to be both cellular age and stress specific. Notably, MV treatment was unable to repair mechanical injury in older co-cultures but remained therapeutic following genotoxic stress. These observations were further confirmed in human dermal fibroblast (HDF) and vascular smooth muscle cell (VSMC) co-cultures of increasing cellular age. In a model of comorbidity comprising co-cultures of HDFs and highly senescent abdominal aortic aneurysm (AAA) VSMCs, MV administration appeared to be senotherapeutic, following both mechanical and genotoxic stress. Our data provide insights into EVs and the specific roles they play during tissue repair and ageing. These data will potentiate the development of novel cell-free therapeutic interventions capable of attenuating age-associated morbidities and avoiding undesired effects.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | McGuinness, Dr Dagmara and Selman, Professor Colin and Panagiotou, Dr Nikolaos and Shiels, Professor Paul |
| Authors: | Panagiotou, N., McGuinness, D., Jaminon, A. M.G., Mees, B., Selman, C., Schurgers, L., and Shiels, P. G. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Chemistry |
| Journal Name: | Cells |
| Publisher: | MDPI |
| ISSN: | 2073-4409 |
| ISSN (Online): | 2073-4409 |
| Published Online: | 23 June 2023 |
| Copyright Holders: | Copyright © 2023 The Authors |
| First Published: | First published in Cells 12(13): 1707 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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