Kazlauskaite, R. et al. (2022) Deploying an in vitro gut model to assay the impact of the mannan-oligosaccharide prebiotic bio-mos on the Atlantic Salmon (Salmo salar) gut microbiome. Microbiology Spectrum, 10(3), e0195321. (doi: 10.1128/spectrum.01953-21) (PMID:35532227) (PMCID:PMC9241627)
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Abstract
Alpha mannose-oligosaccharide (MOS) prebiotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and their associated metabolism. Bio-Mos is a commercially available prebiotic currently used in the agri-feed industry, but studies show contrasting results of its effect on fish performance and feed efficiency. Thus, detailed studies are needed to investigate the effect of MOS supplements on the fish microbiome to enhance our understanding of the link between MOS and gut health. To assess Bio-Mos for potential use as a prebiotic growth promoter in salmonid aquaculture, we have modified an established Atlantic salmon in vitro gut model, SalmoSim, to evaluate its impact on the host microbial communities. The microbial communities obtained from ceca compartments from four adult farmed salmon were inoculated in biological triplicate reactors in SalmoSim. Prebiotic treatment was supplemented for 20 days, followed by a 6-day washout period. Inclusion of Bio-Mos in the media resulted in a significant increase in formate (P = 0.001), propionate (P = 0.037) and 3-methyl butanoic acid (P = 0.024) levels, correlated with increased abundances of several, principally, anaerobic microbial genera (Fusobacterium, Agarivorans, Pseudoalteromonas). DNA metabarcoding with the 16S rDNA marker confirmed a significant shift in microbial community composition in response to Bio-Mos supplementation with observed increase in lactic acid producing Carnobacterium. In conjunction with previous in vivo studies linking enhanced volatile fatty acid production alongside MOS supplementation to host growth and performance, our data suggest that Bio-Mos may be of value in salmonid production. Furthermore, our data highlights the potential role of in vitro gut models to complementin vivo trials of microbiome modulators.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Heys, Dr Chloe and Kazlauskaite, Raminta and Llewellyn, Professor Martin and Cheaib, Dr Bachar and Sloan, Professor William and Russell, Mrs Julie and Humble, Joseph and Ijaz, Dr Umer and Connelly, Dr Stephanie |
| Authors: | Kazlauskaite, R., Cheaib, B., Humble, J., Heys, C., Ijaz, U. Z., Connelly, S., Sloan, W. T., Russell, J., Martinez-Rubio, L., Sweetman, J., Kitts, A., McGinnity, P., Lyons, P., and Llewellyn, M. S. |
| College/School: | College of Science and Engineering > School of Engineering > Infrastructure and Environment College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Microbiology Spectrum |
| Publisher: | American Society for Microbiology |
| ISSN: | 2165-0497 |
| ISSN (Online): | 2165-0497 |
| Published Online: | 09 May 2022 |
| Copyright Holders: | Copyright © 2022 Kazlauskaite et al. |
| First Published: | First published in Microbiology Spectrum 10(3): e0195321 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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