Davies, R. L. and Lee, I. (2006) Diversity of temperate bacteriophages induced in bovine and ovine Mannheimia haemolytica isolates and identification of a new P2-like phage. FEMS Microbiology Letters, 260(2), pp. 162-170. (doi: 10.1111/j.1574-6968.2006.00314.x)
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Publisher's URL: http://dx.doi.org/10.1111/j.1574-6968.2006.00314.x
Abstract
The diversity of temperate bacteriophages was examined in 32 <i>Mannheimia haemolytica</i>, six <i>Mannheimia glucosidal</i> and four <i>Pasteurella trehalosi</i> isolates. Phage particles were induced and identified by electron microscopy in 24 (75%) <i>M. Haemolytica</i> isolates, but in only one (17%) <i>M. Glucosidal</i> and one (25%) <i>P. trehalosi</i> isolate. The <i>M. Haemolytica</i> phages were relatively diverse as seven <i>Siphoviridae</i>, 15 <i>Myoviridae</i> and two <i>Podoviridae</i>-like phages were identified; the <i>Myoviridae</i>-type phages also exhibited structural variation of their tails. The bacteriophages induced in <i>M. Glucosidal</i> and <i>P. trehalosi</i> were of the <i>Myoviridae</i> type. Restriction endonuclease (RE) analysis identified nine distinct RE types among the <i>M. Haemolytica</i> bacteriophages, providing further evidence of their relative diversity. A limited number of phages caused plaques on indicator strains and the phages exhibited a narrow host range. A subgroup of 11 bovine serotype A1 and A6 isolates contained <i>Myoviridae</i>-type phages of the same RE type (type A), but these differed in their abilities to infect and form plaques on the same panel of indicator strains. A P2-like phage (φPHL213.1), representative of the RE type A phages, was identified from the incomplete <i>M. Haemolytica</i> genome sequence. The φPHL213.1 genome contains previously unidentified genes and represents a new member of the P2 phage family.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Davies, Dr Robert |
| Authors: | Davies, R. L., and Lee, I. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | FEMS Microbiology Letters |
| Publisher: | Wiley |
| ISSN: | 0378-1097 |
| ISSN (Online): | 1574-6968 |
| Published Online: | 01 June 2006 |
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