Impact of the biopsy forceps size on histological analysis and performances of the histological scoring systems

Bianchi, E., Najm, A. , Vanbelle, S., Le Goff, B., Mutijima, E., Kaiser, M.-J., Malaise, M. and Hauzeur, J.-P. (2022) Impact of the biopsy forceps size on histological analysis and performances of the histological scoring systems. Scientific Reports, 12, 5692. (doi: 10.1038/s41598-022-09704-w) (PMID:35383240) (PMCID:PMC8983678)

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Abstract

To improve the reliability of the quantitative scorings of the synovial biopsies, we evaluate whether diameter of arthroscopic forceps influences histological quality of synovial tissue and/or histological scores and we compare the intra- and inter-observer performances of the main histological scoring systems. Synovial biopsies were retrieved in the same part of the joint using 1, 2 and 4 mm diameters grasping forceps. After standard staining and immunohistochemistry with anti-CD68 antibody, slides were scored blindly by 2 independent experienced operators for tissue quality and with Krenn score, de Bois-Tak score and CD68 semi-quantitative score. Four samples did not pass quality control. No difference other than a higher number of vessels in the 4 mm versus 2 mm forceps (p = 0.01) was found among the 3 groups. CD68 score was significantly higher in the 2 versus 4 mm forceps (p = 0.009). So we concluded that only vessels quantification and CD68 semi-quantitative score seemed affected by the forceps size. The intra-reader agreement was variable across observers and features: 0.78 (0.66–0.87) for the Krenn scoring system, 0.89 (0.78–0.97) for the de Bois-Tak score and 0.93 (0.81–1.00) for the CD68 score. Interobserver reliabilities of Krenn score, de Bois-Tak score and CD68 scores were satisfactory: 0.95 (0.92–0.99) for Krenn, 0.98 (0.96–0.99) for de Bois-Tak and 0.80 (0.71–0.89) for CD68.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Najm, Dr Aurelie
Authors: Bianchi, E., Najm, A., Vanbelle, S., Le Goff, B., Mutijima, E., Kaiser, M.-J., Malaise, M., and Hauzeur, J.-P.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Research Centre:College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology
Journal Name:Scientific Reports
Publisher:Nature Research
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2022 The Authors
First Published:First published in Scientific Reports 12(1):5692
Publisher Policy:Reproduced under a Creative Commons License

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