Identification and validation of Toxoplasma gondii mitoribosomal large subunit components

Shikha, S., Ferreira Silva, M. and Sheiner, L. (2022) Identification and validation of Toxoplasma gondii mitoribosomal large subunit components. Microorganisms, 10(5), 863. (doi: 10.3390/microorganisms10050863) (PMID:35630308) (PMCID:PMC9145746)

Abstract

Mitochondrial ribosomes are fundamental to mitochondrial function, and thus survival, of nearly all eukaryotes. Despite their common ancestry, mitoribosomes have evolved divergent features in different eukaryotic lineages. In apicomplexans, the mitochondrial rRNA is extremely fragmented raising questions about its evolution, protein composition and structure. Apicomplexan mitochondrial translation and the mitoribosomes are essential in all parasites and life stages studied, highlighting mitoribosomes as a promising target for drugs. Still, the apicomplexan mitoribosome is understudied, with one of the obstacles being that its composition is unknown. Here, to facilitate the study of apicomplexan mitoribosomes, we identified and validated components of the mitoribosomal large subunit in the model apicomplexan Toxoplasma gondii.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Ferreira Silva, Miss Mariana and Sheiner, Professor Lilach

Creator Roles

Ferreira Silva, M.: Methodology, Formal analysis, Writing – review and editing
Sheiner, L.: Conceptualization, Writing – original draft, Writing – review and editing, Supervision, Project administration, Funding acquisition

• Authors: Shikha, S., Ferreira Silva, M., and Sheiner, L.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Microorganisms
• Publisher: MDPI
• ISSN: 2076-2607
• ISSN (Online): 2076-2607
• Published Online: 21 April 2022
• Copyright Holders: Copyright © 2022 The Authors
• First Published: First published in Microorganisms 10(5): 863
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
307056		Lost in translation. A study of the divergent mitochondrial translation pathway of the parasite Toxoplasma gondii	Lilach Sheiner	Wellcome Trust (WELLCOTR)	217173/Z/19/Z	MVLS - Polyomics Facility