Rangarajan, R., Bei, A., Henry, N., Madamet, M., Parzy, D., Nivez, M.P., Doerig, C. and Sultan, A. (2006) Pbcrk-1, the Plasmodium berghei orthologue of P-falciparum cdc-2 related kinase-1 (Pfcrk-1), is essential for completion of the intraerythrocytic asexual cycle. Experimental Parasitology, 112(3), pp. 202-207. (doi: 10.1016/j.exppara.2005.11.002)
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Abstract
The molecular mechanisms underlying gametocytogenesis in malaria parasites are not understood. Plasmodium falciparum cdc2-related kinase 1 (pfcrk-1), a gene that is expressed predominantly in gametocytes, bears homology to the PITSLRE subfamily of cyclin-dependent kinases and has been hypothesized to function as a negative regulator of the cell cycle. We attempted to knock-out pbcrk-1, the P. berghei orthologue of pfcrk-1, but were unable to recover P. berghei parasites with a disrupted pbcrk-1 locus. In contrast, an integration event at this locus that did not result in a loss-of-function of the pbcrk-1 gene was readily observed. This strongly suggests that a functional pbcrk-1 gene product is essential to intraerythrocytic asexual multiplication.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Doerig, Dr Christian |
| Authors: | Rangarajan, R., Bei, A., Henry, N., Madamet, M., Parzy, D., Nivez, M.P., Doerig, C., and Sultan, A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Experimental Parasitology |
| Publisher: | Elsevier |
| ISSN: | 0014-4894 |
| ISSN (Online): | 1090-2449 |
| Published Online: | 20 December 2005 |
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