Spiller, O.B., Goodfellow, I.G., Evans, D.J., Hinchliffe, S.J. and Morgan, B.P. (2002) Coxsackie B viruses that use human DAF as a receptor infect pig cells via pig CAR and do not use pig DAF. Journal of General Virology, 83, pp. 45-52.
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Abstract
Coxsackie B viruses (CVB) are enteroviruses belonging to the family Picornaviridae. Serotypes 1, 3 and 5 of CVB bind to the human membrane complement regulator decay-accelerating factor (DAF) and the coxsackievirus/adenovirus receptor (CAR), using either or both as receptors. These viruses are known to infect pig cell lines, but the receptor(s) involved has not been identified. We have recently characterized the pig homologue of DAF and here explore the interactions of human DAF-binding CVB with pig homologues of DAF and CAR. CVB infection of three pig cell lines resulted in cytolysis, which could be not be blocked by anti-pig DAF antibodies. CVB bound to CHO cells transfected with human DAF, but not pig DAF. Modification of pig DAF by incorporation of the fourth short consensus repeat of human DAF did not confer CVB-binding capacity. CVB did bind CHO cells expressing pig or human CAR, and pre-incubation of pig cells with anti-CAR antibody blocked CVB infection.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Morgan, Prof Brian |
| Authors: | Spiller, O.B., Goodfellow, I.G., Evans, D.J., Hinchliffe, S.J., and Morgan, B.P. |
| College/School: | College of Medical Veterinary and Life Sciences |
| Journal Name: | Journal of General Virology |
| Publisher: | Society for General Microbiology |
| ISSN: | 0022-1317 |
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