The role of accelerated growth plate fusion in the absence of SOCS2 on osteoarthritis vulnerability

Samvelyan, H. J., Huesa, C., Cui, L., Farquharson, C. and Staines, K. A. (2022) The role of accelerated growth plate fusion in the absence of SOCS2 on osteoarthritis vulnerability. Bone and Joint Research, 11(3), pp. 162-170. (doi: 10.1302/2046-3758.113.bjr-2021-0259.r1) (PMID:35272487) (PMCID:PMC8962856)

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Aims: Osteoarthritis (OA) is the most prevalent systemic musculoskeletal disorder, characterized by articular cartilage degeneration and subchondral bone (SCB) sclerosis. Here, we sought to examine the contribution of accelerated growth to OA development using a murine model of excessive longitudinal growth. Suppressor of cytokine signalling 2 (SOCS2) is a negative regulator of growth hormone (GH) signalling, thus mice deficient in SOCS2 (Socs2-/-) display accelerated bone growth. Methods: We examined vulnerability of Socs2-/- mice to OA following surgical induction of disease (destabilization of the medial meniscus (DMM)), and with ageing, by histology and micro-CT. Results: We observed a significant increase in mean number (wild-type (WT) DMM: 532 (SD 56); WT sham: 495 (SD 45); knockout (KO) DMM: 169 (SD 49); KO sham: 187 (SD 56); p < 0.001) and density (WT DMM: 2.2 (SD 0.9); WT sham: 1.2 (SD 0.5); KO DMM: 13.0 (SD 0.5); KO sham: 14.4 (SD 0.7)) of growth plate bridges in Socs2-/- in comparison with WT. Histological examination of WT and Socs2-/- knees revealed articular cartilage damage with DMM in comparison to sham. Articular cartilage lesion severity scores (mean and maximum) were similar in WT and Socs2-/- mice with either DMM, or with ageing. Micro-CT analysis revealed significant decreases in SCB thickness, epiphyseal trabecular number, and thickness in the medial compartment of Socs2-/-, in comparison with WT (p < 0.001). DMM had no effect on the SCB thickness in comparison with sham in either genotype. Conclusion: Together, these data suggest that enhanced GH signalling through SOCS2 deletion accelerates growth plate fusion, however this has no effect on OA vulnerability in this model.

Item Type:Articles
Additional Information:This work was supported by the Medical Research Council (MR/R022240/2) and a University of Brighton Rising Star Award, both to K. A. Staines. C. Farquharson was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) via an Institute Strategic Programme Grant (BB/J004316/1). C. Huesa is supported by an early career fellowship from Versus Arthritis (22483).
Keywords:Orthopedics and Sports Medicine, Surgery
Glasgow Author(s) Enlighten ID:Huesa, Dr Carmen
Authors: Samvelyan, H. J., Huesa, C., Cui, L., Farquharson, C., and Staines, K. A.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Research Centre:College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology
Journal Name:Bone and Joint Research
Publisher:British Editorial Society of Bone and Joint Surgery
Published Online:11 March 2022
Copyright Holders:Copyright © 2022 Author(s) et al
First Published:First published in Bone and Joint Research 11(3): 162-170
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
308503Targeting osteoarthritic pain through Proteinase Activated Receptor 2 (PAR2)Carl GoodyearVersus Arthritis (ARTRESUK)22483III - Immunology